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- W2508214013 abstract "The RNA-guided CRISPR/Cas9 endonuclease and the endonuclease-dead dCas9 protein are powerful genomic manipulation tools for gene editing, transcriptional regulation, and epigenetic modifications. Both Cas9 and dCas9 enable diverse types of high-throughput screening of gene functions in cell lines and in vivo. The CRISPR/Cas9 accelerates the establishment of many useful transgenic animal models for biomedical research. The CRISPR/Cas9 is repurposed for genomic imaging and lineage tracing in living cells and tissues. Recent advances in genome engineering are starting a revolution in biological research and translational applications. The clustered regularly interspaced short palindromic repeats (CRISPR)-associated RNA-guided endonuclease CRISPR associated protein 9 (Cas9) and its variants enable diverse manipulations of genome function. In this review, we describe the development of Cas9 tools for a variety of applications in cell biology research, including the study of functional genomics, the creation of transgenic animal models, and genomic imaging. Novel genome engineering methods offer a new avenue to understand the causality between the genome and phenotype, thus promising a fuller understanding of cell biology. Recent advances in genome engineering are starting a revolution in biological research and translational applications. The clustered regularly interspaced short palindromic repeats (CRISPR)-associated RNA-guided endonuclease CRISPR associated protein 9 (Cas9) and its variants enable diverse manipulations of genome function. In this review, we describe the development of Cas9 tools for a variety of applications in cell biology research, including the study of functional genomics, the creation of transgenic animal models, and genomic imaging. Novel genome engineering methods offer a new avenue to understand the causality between the genome and phenotype, thus promising a fuller understanding of cell biology." @default.
- W2508214013 created "2016-09-16" @default.
- W2508214013 creator A5037788360 @default.
- W2508214013 creator A5064548216 @default.
- W2508214013 date "2016-11-01" @default.
- W2508214013 modified "2023-10-07" @default.
- W2508214013 title "Applications of CRISPR Genome Engineering in Cell Biology" @default.
- W2508214013 cites W1179143542 @default.
- W2508214013 cites W1635615463 @default.
- W2508214013 cites W1636205509 @default.
- W2508214013 cites W1824346003 @default.
- W2508214013 cites W1906155566 @default.
- W2508214013 cites W1916572066 @default.
- W2508214013 cites W1932780354 @default.
- W2508214013 cites W1933893097 @default.
- W2508214013 cites W1947959231 @default.
- W2508214013 cites W1962249777 @default.
- W2508214013 cites W1968931043 @default.
- W2508214013 cites W1972476556 @default.
- W2508214013 cites W1972759536 @default.
- W2508214013 cites W1973260880 @default.
- W2508214013 cites W1976391205 @default.
- W2508214013 cites W1976404496 @default.
- W2508214013 cites W1981160614 @default.
- W2508214013 cites W1982831423 @default.
- W2508214013 cites W1982873976 @default.
- W2508214013 cites W1983197357 @default.
- W2508214013 cites W2001497035 @default.
- W2508214013 cites W2003171404 @default.
- W2508214013 cites W2003797386 @default.
- W2508214013 cites W2007818617 @default.
- W2508214013 cites W2009576434 @default.
- W2508214013 cites W2009626008 @default.
- W2508214013 cites W2010986636 @default.
- W2508214013 cites W2017224987 @default.
- W2508214013 cites W2017381606 @default.
- W2508214013 cites W2019670881 @default.
- W2508214013 cites W2026520402 @default.
- W2508214013 cites W2032464841 @default.
- W2508214013 cites W2033253509 @default.
- W2508214013 cites W2034048377 @default.
- W2508214013 cites W2035420256 @default.
- W2508214013 cites W2036176224 @default.
- W2508214013 cites W2039731535 @default.
- W2508214013 cites W2043890495 @default.
- W2508214013 cites W2043972484 @default.
- W2508214013 cites W2044741494 @default.
- W2508214013 cites W2045435533 @default.
- W2508214013 cites W2047703458 @default.
- W2508214013 cites W2055790209 @default.
- W2508214013 cites W2056512830 @default.
- W2508214013 cites W2061650108 @default.
- W2508214013 cites W2063148079 @default.
- W2508214013 cites W2064815984 @default.
- W2508214013 cites W2066783444 @default.
- W2508214013 cites W2068427957 @default.
- W2508214013 cites W2070134501 @default.
- W2508214013 cites W2070614858 @default.
- W2508214013 cites W2072051684 @default.
- W2508214013 cites W2075230442 @default.
- W2508214013 cites W2077659966 @default.
- W2508214013 cites W2092806325 @default.
- W2508214013 cites W2095950258 @default.
- W2508214013 cites W2099062342 @default.
- W2508214013 cites W2101749257 @default.
- W2508214013 cites W2102526584 @default.
- W2508214013 cites W2103081715 @default.
- W2508214013 cites W2104091091 @default.
- W2508214013 cites W2106296224 @default.
- W2508214013 cites W2107959600 @default.
- W2508214013 cites W2108038398 @default.
- W2508214013 cites W2111648798 @default.
- W2508214013 cites W2112006537 @default.
- W2508214013 cites W2112295554 @default.
- W2508214013 cites W2112444085 @default.
- W2508214013 cites W2113446817 @default.
- W2508214013 cites W2119771879 @default.
- W2508214013 cites W2120503144 @default.
- W2508214013 cites W2122941722 @default.
- W2508214013 cites W2123746851 @default.
- W2508214013 cites W2127749855 @default.
- W2508214013 cites W2127862779 @default.
- W2508214013 cites W2133702185 @default.
- W2508214013 cites W2133908827 @default.
- W2508214013 cites W2140494588 @default.
- W2508214013 cites W2140754284 @default.
- W2508214013 cites W2143772974 @default.
- W2508214013 cites W2147511377 @default.
- W2508214013 cites W2152505144 @default.
- W2508214013 cites W2153344788 @default.
- W2508214013 cites W2153537688 @default.
- W2508214013 cites W2154283358 @default.
- W2508214013 cites W2156319424 @default.
- W2508214013 cites W2160051211 @default.
- W2508214013 cites W2163173465 @default.
- W2508214013 cites W2165869860 @default.
- W2508214013 cites W2167380395 @default.
- W2508214013 cites W2169244146 @default.