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• W2508360430 abstract "Stimuli-responsive activatable reactive tags are applicable to selective labeling of biomolecules in a defined area or environment in living systems, yielding new insights into cellular processes through molecular imaging and fishing. Here, we developed a chemically activatable alkyne tag that can be incorporated into biological molecules and labeled with azide-tagged functional molecules through the alkyne-azide cycloaddition click reaction after chemical activation. Formation of the alkyne tag from the precursor moiety was confirmed to proceed in physiological aqueous media and was particularly enhanced under mildly acidic pH. The tag was successfully applied to low-pH sensitive labeling of a cholesterol analogue with azide-tagged biotin on living mammalian cells. Our results provided proof of principle that the present activatable alkyne tag can be used as a tool to selectively analyze molecules of interest in low-pH regions in living systems." @default.
• W2508360430 created "2016-09-16" @default.
• W2508360430 creator A5005290963 @default.
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• W2508360430 creator A5067928470 @default.
• W2508360430 date "2016-08-23" @default.
• W2508360430 modified "2023-10-01" @default.
• W2508360430 title "Chemically Activatable Alkyne Tag for Low pH-Enhanced Molecular Labeling on Living Cells" @default.
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• W2508360430 doi "https://doi.org/10.1021/acs.bioconjchem.6b00399" @default.
• W2508360430 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27526276" @default.
• W2508360430 hasPublicationYear "2016" @default.
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