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• W2508703457 abstract "To improve the yield of cellulolytic enzyme lignin (CEL) and to minimize the structural changes induced by isolation, the dissolution-regeneration of ball-milled soybean stem in a lithium chloride (LiCl)/dimethyl sulfoxide (DMSO) solvent was applied prior to enzymatic hydrolysis. The regenerated stem was enzymatically hydrolyzed for 72 h to remove the carbohydrates and then extracted with 1, 4-dioxane to obtain the cellulolytic enzyme lignin (named as DR-CEL). As expected, the yield of DR-CEL was much higher than that of CEL. With 2 h of ball-milling, the yield of DR-CEL was 40.1% (based on the total lignin in the raw stem). As the ball-milling time increased to 4 h, the yield of DR-CEL improved up to 82.4%, which was much higher than that of CEL with a yield of 26.0%. All the isolated lignin preparations were characterized using FT-IR, 1H NMR and 1H-13C HSQC NMR. The results showed that the main structural characteristics of these two kinds of lignin (CEL & DR-CEL) were similar, except for a slightly higher amount of carbohydrates in DR-CEL. As a result, the combination of the dissolution–regeneration and enzyme hydrolysis of ball-milled sample has a great potential in isolating lignin with higher yield and minimal structural changes." @default.
• W2508703457 created "2016-09-16" @default.
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• W2508703457 date "2016-11-01" @default.
• W2508703457 modified "2023-09-23" @default.
• W2508703457 title "The utilization of soybean straw III: Isolation and characterization of lignin from soybean straw" @default.
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• W2508703457 doi "https://doi.org/10.1016/j.biombioe.2016.07.021" @default.
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