Matches in SemOpenAlex for { <https://semopenalex.org/work/W2508843145> ?p ?o ?g. }
Showing items 1 to 76 of
76
with 100 items per page.
- W2508843145 abstract "This thesis describes the genetic events that can lead to the loss of expression of HLA class 1 on tumour cells. Earlier studies, in colorectal cancer, have shown both complete loss of surface HLA A, B & C expression and loss of single allele products using antibodies recognizing either monomorphic or polymorphic HLA determinants. Data presented demonstrates that complete loss of HLA class 1 expression correlates with mutations in β2 microglobulin. In a study of 52 colorectal cell lines, 8 showed mutations in β2 microglobulin leading to loss or reduced HLA expression. In fresh colorectal tumours 9/147 had mutations in β2 microglobulin, occurring at single or di-nucleotide repeat sequences. From these tumours 71 were analyzed for microsatellite instability, associated with the loss of DNA mismatch repair, and 7 (10%) found to be unstable. β2 microglobulin mutations were identified in 5 of these tumors. Therefore, mutations in β2 microglobulin occur more frequently in mismatch repair defective tumours than in colorectal tumours in general (p<0.01).The colorectal cell line HCA-7 lacks expression of HLA-A*0101 based on studies with the polymorphic antibody 142.2, but the normal B cell line (EVA-1224) from the same patient expresses A*0101. This thesis shows that the A*0101 gene, in HCA-7, contains an insertion of a cytidine in a cytidine repeat sequence in exon 4. This mutated A*0101 gene has the similar sequence to that of a rare A*0104'null' allele reported to lack A*0104 expression.In a model system a β2 microglobulin-HLA-A*0201 construct was transfected, using an ecdysone inducible expression system, into CHO cells and expression shown to be under the control of the inducer, Ponasterone A. Functional studies demonstrated the construct to be capable of presenting the flu virus peptide GILGFVFTL to a clone of human A*0201 restricted cytotoxic T cells and for the T cells to efficiently lyse the CHO cells." @default.
- W2508843145 created "2016-09-16" @default.
- W2508843145 creator A5053392749 @default.
- W2508843145 date "2003-11-21" @default.
- W2508843145 modified "2023-09-27" @default.
- W2508843145 title "Abnormalities of HLA and β₂microglobulin expression on tumour cells" @default.
- W2508843145 doi "https://doi.org/10.21954/ou.ro.0000f57c" @default.
- W2508843145 hasPublicationYear "2003" @default.
- W2508843145 type Work @default.
- W2508843145 sameAs 2508843145 @default.
- W2508843145 citedByCount "0" @default.
- W2508843145 crossrefType "dissertation" @default.
- W2508843145 hasAuthorship W2508843145A5053392749 @default.
- W2508843145 hasConcept C104317684 @default.
- W2508843145 hasConcept C121608353 @default.
- W2508843145 hasConcept C147483822 @default.
- W2508843145 hasConcept C153911025 @default.
- W2508843145 hasConcept C159654299 @default.
- W2508843145 hasConcept C180754005 @default.
- W2508843145 hasConcept C188280979 @default.
- W2508843145 hasConcept C203014093 @default.
- W2508843145 hasConcept C21957315 @default.
- W2508843145 hasConcept C2779767149 @default.
- W2508843145 hasConcept C36823959 @default.
- W2508843145 hasConcept C501734568 @default.
- W2508843145 hasConcept C502942594 @default.
- W2508843145 hasConcept C526805850 @default.
- W2508843145 hasConcept C54355233 @default.
- W2508843145 hasConcept C60748783 @default.
- W2508843145 hasConcept C61320498 @default.
- W2508843145 hasConcept C86803240 @default.
- W2508843145 hasConceptScore W2508843145C104317684 @default.
- W2508843145 hasConceptScore W2508843145C121608353 @default.
- W2508843145 hasConceptScore W2508843145C147483822 @default.
- W2508843145 hasConceptScore W2508843145C153911025 @default.
- W2508843145 hasConceptScore W2508843145C159654299 @default.
- W2508843145 hasConceptScore W2508843145C180754005 @default.
- W2508843145 hasConceptScore W2508843145C188280979 @default.
- W2508843145 hasConceptScore W2508843145C203014093 @default.
- W2508843145 hasConceptScore W2508843145C21957315 @default.
- W2508843145 hasConceptScore W2508843145C2779767149 @default.
- W2508843145 hasConceptScore W2508843145C36823959 @default.
- W2508843145 hasConceptScore W2508843145C501734568 @default.
- W2508843145 hasConceptScore W2508843145C502942594 @default.
- W2508843145 hasConceptScore W2508843145C526805850 @default.
- W2508843145 hasConceptScore W2508843145C54355233 @default.
- W2508843145 hasConceptScore W2508843145C60748783 @default.
- W2508843145 hasConceptScore W2508843145C61320498 @default.
- W2508843145 hasConceptScore W2508843145C86803240 @default.
- W2508843145 hasLocation W25088431451 @default.
- W2508843145 hasOpenAccess W2508843145 @default.
- W2508843145 hasPrimaryLocation W25088431451 @default.
- W2508843145 hasRelatedWork W1977495045 @default.
- W2508843145 hasRelatedWork W2054473251 @default.
- W2508843145 hasRelatedWork W2056168912 @default.
- W2508843145 hasRelatedWork W2058043554 @default.
- W2508843145 hasRelatedWork W2068537935 @default.
- W2508843145 hasRelatedWork W2087807885 @default.
- W2508843145 hasRelatedWork W2089544326 @default.
- W2508843145 hasRelatedWork W2123105305 @default.
- W2508843145 hasRelatedWork W2328073382 @default.
- W2508843145 hasRelatedWork W2336061698 @default.
- W2508843145 hasRelatedWork W2609196580 @default.
- W2508843145 hasRelatedWork W2739720253 @default.
- W2508843145 hasRelatedWork W2740391184 @default.
- W2508843145 hasRelatedWork W2758499819 @default.
- W2508843145 hasRelatedWork W3082955524 @default.
- W2508843145 hasRelatedWork W3091590253 @default.
- W2508843145 hasRelatedWork W3136928370 @default.
- W2508843145 hasRelatedWork W3207210287 @default.
- W2508843145 hasRelatedWork W2184888694 @default.
- W2508843145 hasRelatedWork W2546899819 @default.
- W2508843145 isParatext "false" @default.
- W2508843145 isRetracted "false" @default.
- W2508843145 magId "2508843145" @default.
- W2508843145 workType "dissertation" @default.