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• W2509350040 endingPage "8686" @default.
• W2509350040 startingPage "8668" @default.
• W2509350040 abstract "Synaptic vesicle (SV) pools must maintain a functional repertoire of proteins to efficiently release neurotransmitter. The accumulation of old or damaged proteins on SV membranes is linked to synaptic dysfunction and neurodegeneration. However, despite the importance of SV protein turnover for neuronal health, the molecular mechanisms underlying this process are largely unknown. Here, we have used dissociated rat hippocampal neurons to investigate the pathway for SV protein degradation. We find that neuronal activity drives the degradation of a subset of SV proteins and that the endosomal sorting complex required for transport (ESCRT) machinery and SV-associated GTPase Rab35 are key elements of this use-dependent degradative pathway. Specifically, neuronal activity induces Rab35 activation and binding to the ESCRT-0 protein Hrs, which we have identified as a novel Rab35 effector. These actions recruit the downstream ESCRT machinery to SV pools, thereby initiating SV protein degradation via the ESCRT pathway. Our findings show that the Rab35/ESCRT pathway facilitates the activity-dependent removal of specific proteins from SV pools, thereby maintaining presynaptic protein homeostasis.Synaptic transmission is mediated by the release of chemical neurotransmitters from synaptic vesicles (SVs). This tightly regulated process requires a functional pool of SVs, necessitating cellular mechanisms for removing old or damaged proteins that could impair SV cycling. Here, we show that a subset of SV proteins is degraded in an activity-dependent manner and that key steps in this degradative pathway are the activation of the small GTPase Rab35 and the subsequent recruitment of the endosomal sorting complex required for transport (ESCRT) machinery to SV pools. Further, we demonstrate that ESCRT-0 component Hrs is an effector of Rab35, thus providing novel mechanistic insight into the coupling of neuronal activity with SV protein degradation and the maintenance of functional SV pools." @default.
• W2509350040 created "2016-09-16" @default.
• W2509350040 creator A5026386775 @default.
• W2509350040 creator A5032512791 @default.
• W2509350040 creator A5033521896 @default.
• W2509350040 creator A5054228916 @default.
• W2509350040 creator A5086505100 @default.
• W2509350040 date "2016-08-17" @default.
• W2509350040 modified "2023-10-10" @default.
• W2509350040 title "Activity-Dependent Degradation of Synaptic Vesicle Proteins Requires Rab35 and the ESCRT Pathway" @default.
• W2509350040 cites W1484359485 @default.
• W2509350040 cites W1486436311 @default.
• W2509350040 cites W1493735997 @default.
• W2509350040 cites W1495816175 @default.
• W2509350040 cites W1513729579 @default.
• W2509350040 cites W1635662028 @default.
• W2509350040 cites W1670369629 @default.
• W2509350040 cites W1876590017 @default.
• W2509350040 cites W1970275852 @default.
• W2509350040 cites W1973949147 @default.
• W2509350040 cites W1974359647 @default.
• W2509350040 cites W1976161883 @default.
• W2509350040 cites W1976543990 @default.
• W2509350040 cites W1982139859 @default.
• W2509350040 cites W1987151296 @default.
• W2509350040 cites W1988178943 @default.
• W2509350040 cites W1990587513 @default.
• W2509350040 cites W1994668150 @default.
• W2509350040 cites W1996669272 @default.
• W2509350040 cites W1997603072 @default.
• W2509350040 cites W1998079639 @default.
• W2509350040 cites W1999371762 @default.
• W2509350040 cites W2000874341 @default.
• W2509350040 cites W2001428953 @default.
• W2509350040 cites W2012973109 @default.
• W2509350040 cites W2013022391 @default.
• W2509350040 cites W2017040646 @default.
• W2509350040 cites W2017726369 @default.
• W2509350040 cites W2030773336 @default.
• W2509350040 cites W2032377673 @default.
• W2509350040 cites W2032516003 @default.
• W2509350040 cites W2035381521 @default.
• W2509350040 cites W2037174767 @default.
• W2509350040 cites W2039919932 @default.
• W2509350040 cites W2040729638 @default.
• W2509350040 cites W2042884097 @default.
• W2509350040 cites W2046844445 @default.
• W2509350040 cites W2048030072 @default.
• W2509350040 cites W2048878540 @default.
• W2509350040 cites W2049878966 @default.
• W2509350040 cites W2050606229 @default.
• W2509350040 cites W2053129791 @default.
• W2509350040 cites W2064862804 @default.
• W2509350040 cites W2064949318 @default.
• W2509350040 cites W2066983405 @default.
• W2509350040 cites W2067071342 @default.
• W2509350040 cites W2070709903 @default.
• W2509350040 cites W2074532659 @default.
• W2509350040 cites W2075677669 @default.
• W2509350040 cites W2079398298 @default.
• W2509350040 cites W2080967954 @default.
• W2509350040 cites W2089778398 @default.
• W2509350040 cites W2096575464 @default.
• W2509350040 cites W2101675738 @default.
• W2509350040 cites W2102174960 @default.
• W2509350040 cites W2103527253 @default.
• W2509350040 cites W2105593801 @default.
• W2509350040 cites W2105895439 @default.
• W2509350040 cites W2112001589 @default.
• W2509350040 cites W2113104319 @default.
• W2509350040 cites W2115392798 @default.
• W2509350040 cites W2120920292 @default.
• W2509350040 cites W2122511788 @default.
• W2509350040 cites W2124433234 @default.
• W2509350040 cites W2128208230 @default.
• W2509350040 cites W2128573075 @default.
• W2509350040 cites W2131097779 @default.
• W2509350040 cites W2131997549 @default.
• W2509350040 cites W2144447780 @default.
• W2509350040 cites W2147257825 @default.
• W2509350040 cites W2153078270 @default.
• W2509350040 cites W2153552926 @default.
• W2509350040 cites W2163324763 @default.
• W2509350040 cites W2164646446 @default.
• W2509350040 cites W2164899453 @default.
• W2509350040 cites W2181193101 @default.
• W2509350040 cites W2261583454 @default.
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• W2509350040 doi "https://doi.org/10.1523/jneurosci.0725-16.2016" @default.
• W2509350040 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4987437" @default.
• W2509350040 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27535913" @default.
• W2509350040 hasPublicationYear "2016" @default.
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