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- W2509771320 abstract "It was previously reported that the brown colour pigment in roast coffee infusion was separated into main three components(A, B, and C)by Sephadex G-25 column chromatography. To clarify the precursors of these brown pigments, the following model roast method was designed. Pack the mixture of 2ml of test solution(pH5.5)and 1.5g of Celite hollowly in a test tube and heat at 190°C for 30 minutes. Add 6ml of water, warm in a boiling bath, cool and centrifuge. Brown pigments in supernatant are separated and estimated by column chromatography. Among many fractions of coffee beans extract separated by solvent extraction, lead acetate precipitation and ion exchange resin chromatography, the fraction containing sugar and chlorogenic acids produced three brown pigments. Sugars gave pigment A and a small amount of B, while chlorogenic acid did not. But when chlorogenic acid coexisted sugars, sugar alcohols, or arabinogalactan of coffee beans gave three pigments. Amino acid and caffein had no effect. In the same way, ether and ethyl acetate extracts from roasted sucrose, and glycerin aldehyde, glycol aldehyde and 5-hydroxymethylfurfrual gave three pigments.From these results, formation mechanism of these brown pigments in coffee was proposed as follows:" @default.
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- W2509771320 doi "https://doi.org/10.3136/nskkk1962.24.124" @default.
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