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- W2510133805 abstract "Protein modification with the small ubiquitin-related modifier SUMO is a potent regulatory mechanism implicated in a variety of biological pathways. In vitro sumoylation reactions have emerged as a versatile tool to identify and characterize novel SUMO enzymes as well as their substrates. Here, we present detailed protocols for the purification and fluorescent labeling of mammalian SUMO paralogs for their application in sumoylation assays. These assays provide a fast readout for in vitro SUMO chain formation activity of E3 ligases in a paralog-specific manner. Finally, we critically analyze the application of fluorescent SUMO proteins to study substrate modification in vitro revealing also the drawbacks of the system." @default.
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- W2510133805 date "2016-01-01" @default.
- W2510133805 modified "2023-09-25" @default.
- W2510133805 title "A Fluorescent In Vitro Assay to Investigate Paralog-Specific SUMO Conjugation" @default.
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- W2510133805 doi "https://doi.org/10.1007/978-1-4939-6358-4_5" @default.
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