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• W2510364534 endingPage "e1006278" @default.
• W2510364534 startingPage "e1006278" @default.
• W2510364534 abstract "Patterning of C. elegans vulval cell fates relies on inductive signaling. In this induction event, a single cell, the gonadal anchor cell, secretes LIN-3/EGF and induces three out of six competent precursor cells to acquire a vulval fate. We previously showed that this developmental system is robust to a four-fold variation in lin-3/EGF genetic dose. Here using single-molecule FISH, we find that the mean level of expression of lin-3 in the anchor cell is remarkably conserved. No change in lin-3 expression level could be detected among C. elegans wild isolates and only a low level of change—less than 30%—in the Caenorhabditis genus and in Oscheius tipulae. In C. elegans, lin-3 expression in the anchor cell is known to require three transcription factor binding sites, specifically two E-boxes and a nuclear-hormone-receptor (NHR) binding site. Mutation of any of these three elements in C. elegans results in a dramatic decrease in lin-3 expression. Yet only a single E-box is found in the Drosophilae supergroup of Caenorhabditis species, including C. angaria, while the NHR-binding site likely only evolved at the base of the Elegans group. We find that a transgene from C. angaria bearing a single E-box is sufficient for normal expression in C. elegans. Even a short 58 bp cis-regulatory fragment from C. angaria with this single E-box is able to replace the three transcription factor binding sites at the endogenous C. elegans lin-3 locus, resulting in the wild-type expression level. Thus, regulatory evolution occurring in cis within a 58 bp lin-3 fragment, results in a strict requirement for the NHR binding site and a second E-box in C. elegans. This single-cell, single-molecule, quantitative and functional evo-devo study demonstrates that conserved expression levels can hide extensive change in cis-regulatory site requirements and highlights the evolution of new cis-regulatory elements required for cell-specific gene expression." @default.
• W2510364534 created "2016-09-16" @default.
• W2510364534 creator A5036280156 @default.
• W2510364534 creator A5053710443 @default.
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• W2510364534 creator A5089086138 @default.
• W2510364534 date "2016-09-02" @default.
• W2510364534 modified "2023-10-18" @default.
• W2510364534 title "Evolution of New cis-Regulatory Motifs Required for Cell-Specific Gene Expression in Caenorhabditis" @default.
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• W2510364534 cites W1944127002 @default.
• W2510364534 cites W1963690635 @default.
• W2510364534 cites W1964733653 @default.
• W2510364534 cites W1966781601 @default.
• W2510364534 cites W1968189089 @default.
• W2510364534 cites W1968763631 @default.
• W2510364534 cites W1974744327 @default.
• W2510364534 cites W1977561367 @default.
• W2510364534 cites W1980834769 @default.
• W2510364534 cites W1981026612 @default.
• W2510364534 cites W1981346316 @default.
• W2510364534 cites W1985237887 @default.
• W2510364534 cites W1985922092 @default.
• W2510364534 cites W1988865037 @default.
• W2510364534 cites W1991063874 @default.
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• W2510364534 cites W1999192371 @default.
• W2510364534 cites W2008126806 @default.
• W2510364534 cites W2009975822 @default.
• W2510364534 cites W2015202087 @default.
• W2510364534 cites W2020766456 @default.
• W2510364534 cites W2021312680 @default.
• W2510364534 cites W2025665146 @default.
• W2510364534 cites W2027120339 @default.
• W2510364534 cites W2027237084 @default.
• W2510364534 cites W2029436624 @default.
• W2510364534 cites W2037652809 @default.
• W2510364534 cites W2044726214 @default.
• W2510364534 cites W2045645277 @default.
• W2510364534 cites W2046727187 @default.
• W2510364534 cites W2051872662 @default.
• W2510364534 cites W2053016349 @default.
• W2510364534 cites W2060297895 @default.
• W2510364534 cites W2062624102 @default.
• W2510364534 cites W2063805004 @default.
• W2510364534 cites W2064691827 @default.
• W2510364534 cites W2065508021 @default.
• W2510364534 cites W2066461878 @default.
• W2510364534 cites W2066908868 @default.
• W2510364534 cites W2074167843 @default.
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• W2510364534 cites W2088990426 @default.
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• W2510364534 cites W2170739826 @default.
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• W2510364534 doi "https://doi.org/10.1371/journal.pgen.1006278" @default.
• W2510364534 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5010242" @default.
• W2510364534 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27588814" @default.
• W2510364534 hasPublicationYear "2016" @default.
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