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- W25109030 abstract "Using a model mouse hybridoma cell line in serum-free continuous culture experiments we explored the influence of the feed medium composition on cell numbers, extra- and intracellular metabolite concentrations, substrate and byproduct consumption/production rates, and yield coefficients as a function of step changes in glutamine and Primatone RL (enzymatic meat hydrolyzate, i.e., source of added essential and non-essential amino acids). In addition to analyzing the culture supernatants for steady-state and transient responses upon changes in the medium composition, perchloric acid extracts of cells were prepared and significant differences in amount and composition of intracellular amino acids were noted. Increased antibody production rates were found to be associated with increased intracellular amino acid concentrations." @default.
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- W25109030 date "1992-01-01" @default.
- W25109030 modified "2023-09-27" @default.
- W25109030 title "CONTINUOUS HYBRIDOMA SUSPENSION CULTURES: CHANGES IN FEED MEDIUM COMPOSITION AND THEIR EFFECTS ON EXTRACELLULAR AND INTRACELLULAR METABOLITE CONCENTRATIONS" @default.
- W25109030 doi "https://doi.org/10.1016/b978-0-7506-0421-5.50074-x" @default.
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