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- W2511233979 abstract "[Purpose] The non-equilibrium atmospheric pressure plasma alters various cellular functions such as the membrane transport1 and the intracellular signaling2. While the plasma has recently emerged as a tool in cancer therapy, the mechanisms remain to be determined in comprehensive and quantitative manners. The purpose of this study was thus to investigate the plasma-induced cellular responses using the SWATHTM-based comprehensive quantitative proteomics. [Method] Membrane protein fractions and whole cell lysates were prepared from human colorectal adenocarcinoma Caco-2 cells, which were incubated with the plasma-irradiated physiological buffer or the normal buffer as control. The trypsin-digested peptides were comprehensively quantified with liquid chromatography-mass spectrometry (LC-MS/MS)-based SWATH™ method. The phosphorylated peptides were concentrated by the hydroxy acid-modified metal oxide chromatography followed by dephosphorization. Spectral peptide ion database of various proteomes was const ructed to identify the proteins in the SWATHTM analysis. The peak area intensities of each identified peptide were compared between the plasma-treated and non-treated group. [Results and Discussion] The expression levels of 1468 peptides in the membrane proteins of the plasma-treated Caco-2 cells were significantly changed by over 1.2-fold or less than 0.8-fold (p<0.05). The identified proteins include transport proteins, receptors of macromolecules, and endocytosis-related proteins. T he abundance of phosphorylated 253 peptides was altered in the whole cell lys a t e s of the plasma-treated Caco-2 cells. The levels of phosphorylated peptides were decreased in the epithelial adherens signaling-related proteins. These changes in the phosphorylation profiles were distinguished from those in hydrogen peroxide-treated Caco-2 cells. The results support the belief that the atmospheric-pressure plasma would affect the membrane transport and intracellular signaling by changing the protein expression profiles in the plasma membrane and the phosphorylation levels of intracellular signaling-related proteins." @default.
- W2511233979 created "2016-09-16" @default.
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- W2511233979 date "2016-06-01" @default.
- W2511233979 modified "2023-09-27" @default.
- W2511233979 title "Atmospheric-pressure plasma-induced cellular responses in human colorectal adenocarcinoma Caco-2 cells: A study of comprehensive quantitative proteomics" @default.
- W2511233979 doi "https://doi.org/10.1109/plasma.2016.7534130" @default.
- W2511233979 hasPublicationYear "2016" @default.
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