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- W2511342938 abstract "Grain mould caused by several fungi is a serious disease of sorghum which develops during high humidity and warm temperature conditions. Occurrence of grain mould disease is severe when rainfall coincides with the flowering of the crop. Mould damage reduces seed viability, test weight, nutritional quality and the market value. Use of resistant cultivars is the only economical and feasible method to minimize damage from grain moulds. Not much work has been done on the variability of fungi associated with moulded grains of sorghum which needs special attention, since breeding for resistance is based on the knowledge of existence of variation in the pathogen population. Hence, the present investigation was undertaken with the grain mould samples collected from different sorghum cultivars grown at International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) and National Research Centre for Sorghum (NRCS), Hyderabad fields during Kharif 2002 to study the variability among the isolates of major grain mould fungi. Fungi associated with moulded grains of 12 sorghum cultivars were isolated separately by standard blotter method. The fungal cultures were purified by hyphal tip method. Single spore cultures and hyphal tip isolations of these fungi were maintained on PDA medium by periodic transfers. In all, ten fungal genera were found to be associated with the moulded grains of sorghum cultivars. Among them, Fusarium moniliforme Sheld, Curvularia lunata (Wakker) Boedjin, and Phoma sorghina (Sacc.) Boerema, Dorenbosch and Van Kesteren were predominant which were identified up to species level and these isolates were used to study the cultural, morphological and pathogenic variability. Among different media tested, potato dextrose agar (PDA) was found to be the best medium for the growth of Fusarium moniliforme. While OMA supported maximum growth of Curvularia lunata, whereas, both PDA and OMA found to be the best for the growth of Phoma sorghina. Variations were observed within the isolates of F. moniliforme with respect to the radial growth, growth rate and size of micro and macroconidia. The isolate Fm2 (SPH 1442) recorded maximum growth and growth rate on PDA compared to other isolates. Minimum growth and growth rate was observed in the isolate Fm12 (KR 194). The size of microconidia and macroconidia ranged from 6.11 – 9.24 x 2.30 – 2.83 m and 32.63 – 47.63 x 2.13 – 3.96 m respectively. Maximum length of microconidia was recorded in Fm3 (SPV1609) isolate and minimum length was observed in isolate Fm10 (PVK 801) while maximum breadth of microconidia observed in isolate Fm12 (KR 194) and minimum breadth was in isolate Fm 9 (C-43).Size of macroconidia was maximum in the isolate Fm 4, while it was minimum in isolate Fm5. The isolates Fm5 (CSH 16), Fm7 (SPV 104), Fm 10 (PVK 801) and Fm12 (KR 194) produced sparse growth compared to the other isolates on PDA. All the isolates produced cottony white mycelium with pink substrate pigmentation All the test isolates produced abundant microconidia and few macroconidia when cultured on PDA medium. None of the isolates produced chlamydospores in the medium. The isolates of C. lunata differed significantly in respect of colony diameter, growth rate and size of conidia on OMA medium. Maximum colony diameter and growth rate (90 mm 12.14 mm/day) was recorded in Cl 7 (SPV 104) and Cl 12 (KR 194) isolates and minimum radial growth and growth rate (84.6 mm 11.37 mm/day) was recorded in isolate Cl 1 (SPH 1420). No distinct variation was observed in respect of colony type of the various isolates of C. lunata, which produced dark brown to black colonies on OMA medium. The size of conidia varied significantly within the isolates ranged from 19.00 – 24.26 x 8.73 – 11.80 m. Maximum length and breadth of conidia recorded in isolate Cl8 (AKMS 14B). Minimum breadth and length was recorded in isolates Cl 1 (SPH (1420) and Cl 10 (PVK 801) respectively. Of the twelve isolates of P. sorghina studied maximum radial growth and growth rate (86.80 mm and 11.68 mm day) was observed in isolate Ps11 (KR 188) which was on par with the isolates Ps1 (SPH 1420), Ps6 (GM 950093), Ps7 (SPV 104), Ps8 (AKMS 14B) and Ps10 (PVK 801). While minimum growth and growth rate (78.2 mm and 10.46 mm day) was recorded in ps4 (CSH 14) which was on par with the isolates Ps3 (SPV 1609) and Ps5 (CSH 16). No distinct variations were observed on colony type and colour of the isolates grown on PDA. All the isolates produced dark brown and spherical pycnidia after 15 days of incubation at 25+1C. Size of pycnidia of P. sorghina varied significantly among the test isolates. The isolate Ps6 (GM 950093) produced pycnidia of 243.33 m diameter size while the isolate Ps1 (SPH 1420) produced smallest pycnidia of 140.66 m diameter size. No distinct differences in shape of the pycnidia and conidia were observed among the test isolates. The size of conidia ranged from 5.70 – 7.26 x 2.60 – 3.37 m. Maximum and minimum sized conidia were produced by the isolates Ps6 (GM 950093) and Ps1 (SPH 1420) respectively. Pathogenicity test with the test isolates of F. moniliforme, C.. lunata and P. sorghina showed differential mould reaction on known susceptible and resistant sorghum cultivars. Of the twelve isolates of F. moniliforme tested, isolate Fm 5 (CSH 16) was found to be highly virulent infecting all the ten sorghum test cultivars. Three cultivars viz., DMS 652, IS 14338 and IS 14862 showed resistant reaction to all the test isolates of F. moniliforme except Fm5 and Fm12 Among the twelve isolates of C. lunata tested, isolates viz., Cl 11 (KR 188) and Cl 9 (C43)were found to be highly virulent compared to the Cl 7 (SPV 104) isolate. IS 14338 and DMS 652 cultivars recorded moderately resistant reaction while all other cultivars were highly susceptible to C. lunata. Of the ten sorghum cultivars tested, three cultivars viz., 14338, IS 14862 and DMS 652 were found resistant to P. sorghina infection. Among the test isolates, Ps11 (KR 188) was found to be highly virulent. Significant differences were observed among the mould resistant and susceptible cultivars of sorghum for grain hardness measured by Stenvert hardness tester (SHT). Grain hardness values (SHT time in seconds) were maximum for mould resistant cultivars compared to the susceptible ones. CHAPTER –" @default.
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- W2511342938 date "2004-01-01" @default.
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- W2511342938 title "VARIABILITY OF MAJOR GRAIN MOULD FUNGI OF SORGHUM, Sorghum bicolor (L.) Moench" @default.
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