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- W2511381481 abstract "Molecular hybridization between (3HI- poly(U) and unlabeled RNA prepared from sea urchin eggs and embryos has been used to contrast the subcellular localization as well as the size distribution of adenylylated maternal RNA preexisting in the unfertilized egg with that adenylylated as a function of fertilization. Evidence re- ported establishes that such preadenylylated genetic mes- sages are predominantly located in the ovum's subriboso- mal fraction and that fertilization elicits a rapid realloca- tion of these latent transcripts into the zygote's ribosomal fraction. Examination of the size distribution of the adenylylated RNA further demonstrates that the un- fertilized egg contains a substantial population of RNA transcripts of exceptionally high molecular weight that are used as primers for the 2-fold net synthesis of poly(A) that follows fertilization. The poly(A)-rich tracts are shown to be covalently bonded to RNA. Assessment of the poly(A) content of nuclear and cytoplasmic fractions suggests that the function of poly(A) is not confined to the transport of genetic messages from the nucleus." @default.
- W2511381481 created "2016-09-16" @default.
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- W2511381481 date "2016-01-01" @default.
- W2511381481 modified "2023-09-23" @default.
- W2511381481 title "Cytoplasmic Adenylylation and Processing (sea urchin eggs and embryos/poly(A)/subcellular localiz" @default.
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