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• W2511451674 endingPage "2548" @default.
• W2511451674 startingPage "2536" @default.
• W2511451674 abstract "ABSTRACT Helicobacter pylori must be able to rapidly respond to fluctuating conditions within the stomach. Despite this need for constant adaptation, H. pylori encodes few regulatory proteins. Of the identified regulators, the ferric uptake regulator (Fur), the nickel response regulator (NikR), and the two-component acid response system (ArsRS) are each paramount to the success of this pathogen. While numerous studies have individually examined these regulatory proteins, little is known about their combined effect. Therefore, we constructed a series of isogenic mutant strains that contained all possible single, double, and triple regulatory mutations in Fur, NikR, and ArsS. A growth curve analysis revealed minor variation in growth kinetics across the strains; these were most pronounced in the triple mutant and in strains lacking ArsS. Visual analysis showed that strains lacking ArsS formed large aggregates and a biofilm-like matrix at the air-liquid interface. Biofilm quantification using crystal violet assays and visualization via scanning electron microscopy (SEM) showed that all strains lacking ArsS or containing a nonphosphorylatable form of ArsR (ArsR-D52N mutant) formed significantly more biofilm than the wild-type strain. Molecular characterization of biofilm formation showed that strains containing mutations in the ArsRS pathway displayed increased levels of cell aggregation and adherence, both of which are key to biofilm development. Furthermore, SEM analysis revealed prevalent coccoid cells and extracellular matrix formation in the ArsR-D52N, Δ nikR Δ arsS , and Δ fur Δ nikR Δ arsS mutant strains, suggesting that these strains may have an exacerbated stress response that further contributes to biofilm formation. Thus, H. pylori ArsRS has a previously unrecognized role in biofilm formation. IMPORTANCE Despite a paucity of regulatory proteins, adaptation is key to the survival of H. pylori within the stomach. While prior studies have focused on individual regulatory proteins, such as Fur, NikR, and ArsRS, few studies have examined the combined effect of these factors. Analysis of isogenic mutant strains that contained all possible single, double, and triple regulatory mutations in Fur, NikR, and ArsS revealed a previously unrecognized role for the acid-responsive two-component system ArsRS in biofilm formation." @default.
• W2511451674 created "2016-09-16" @default.
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• W2511451674 date "2016-09-15" @default.
• W2511451674 modified "2023-10-18" @default.
• W2511451674 title "Characterization of Key Helicobacter pylori Regulators Identifies a Role for ArsRS in Biofilm Formation" @default.
• W2511451674 cites W1481707763 @default.
• W2511451674 cites W1713173235 @default.
• W2511451674 cites W1862098312 @default.
• W2511451674 cites W1952640296 @default.
• W2511451674 cites W1967508755 @default.
• W2511451674 cites W1970374612 @default.
• W2511451674 cites W1976184266 @default.
• W2511451674 cites W1976535776 @default.
• W2511451674 cites W1977574307 @default.
• W2511451674 cites W1978105695 @default.
• W2511451674 cites W1981915387 @default.
• W2511451674 cites W1983900236 @default.
• W2511451674 cites W1991030995 @default.
• W2511451674 cites W1995186148 @default.
• W2511451674 cites W2000971200 @default.
• W2511451674 cites W2012503970 @default.
• W2511451674 cites W2014181528 @default.
• W2511451674 cites W2014368385 @default.
• W2511451674 cites W2015871891 @default.
• W2511451674 cites W2016299826 @default.
• W2511451674 cites W2022058108 @default.
• W2511451674 cites W2022069304 @default.
• W2511451674 cites W2029992296 @default.
• W2511451674 cites W2031960470 @default.
• W2511451674 cites W2035135843 @default.
• W2511451674 cites W2050323750 @default.
• W2511451674 cites W2056028355 @default.
• W2511451674 cites W2062132676 @default.
• W2511451674 cites W2087715958 @default.
• W2511451674 cites W2092244712 @default.
• W2511451674 cites W2102713587 @default.
• W2511451674 cites W2104560884 @default.
• W2511451674 cites W2105277740 @default.
• W2511451674 cites W2107710361 @default.
• W2511451674 cites W2109275139 @default.
• W2511451674 cites W2110132014 @default.
• W2511451674 cites W2110219117 @default.
• W2511451674 cites W2112063053 @default.
• W2511451674 cites W2115064806 @default.
• W2511451674 cites W2115422165 @default.
• W2511451674 cites W2120904752 @default.
• W2511451674 cites W2121871451 @default.
• W2511451674 cites W2126480284 @default.
• W2511451674 cites W2127818778 @default.
• W2511451674 cites W2130657724 @default.
• W2511451674 cites W2130977108 @default.
• W2511451674 cites W2133617878 @default.
• W2511451674 cites W2135855581 @default.
• W2511451674 cites W2138166666 @default.
• W2511451674 cites W2138194854 @default.
• W2511451674 cites W2139704756 @default.
• W2511451674 cites W2142100540 @default.
• W2511451674 cites W2145589079 @default.
• W2511451674 cites W2149518927 @default.
• W2511451674 cites W2151395223 @default.
• W2511451674 cites W2155544436 @default.
• W2511451674 cites W2156815540 @default.
• W2511451674 cites W2157826403 @default.
• W2511451674 cites W2158657807 @default.
• W2511451674 cites W2160154396 @default.
• W2511451674 cites W2164157419 @default.
• W2511451674 cites W2164698153 @default.
• W2511451674 cites W2166101181 @default.
• W2511451674 cites W2167286617 @default.
• W2511451674 cites W2167428512 @default.
• W2511451674 cites W2171373491 @default.
• W2511451674 cites W2172177565 @default.
• W2511451674 cites W2183050346 @default.
• W2511451674 cites W2195139930 @default.
• W2511451674 cites W2203974053 @default.
• W2511451674 cites W2293346959 @default.
• W2511451674 cites W4244789261 @default.
• W2511451674 doi "https://doi.org/10.1128/jb.00324-16" @default.
• W2511451674 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4999924" @default.
• W2511451674 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27432830" @default.
• W2511451674 hasPublicationYear "2016" @default.
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