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- W2511852814 abstract "Abstract Aspergillus sp. has great importance in the present scenario in human and plants by producing immunosuppressive secondary metabolites, aflatoxins, carcinogenic, and teratogenic. The diversity of Aspergillus in the present scenario remains poorly understood. The assessment of true biodiversity of Aspergillus and other fungi in nature is essential for the understanding of their biology. There are several methods available for the detection of biodiversity, such as morphological character, biochemical molecules, and molecular molecules. Recent technological advancements have allowed significant progress in documenting fungal diversity. The use of DNA-based techniques, mainly DNA sequencing, for detecting fungi in the environment revolutionized our view of how and where fungi live. The most widely used genomic region for delimiting fungal species is the internal transcribed spacer (ITS), a short DNA segment with enough intraspecific variation that is suitable for diagnosing different species. Because of the high speed, low cost, and significant technical advantages, next-generation sequencing techniques (NGSTs) have been extensively used in fungal diversity in the past decade. Next-generation sequencing (NGS) has enormous potential to further our understanding of microbial biodiversity. In addition, NGSTs eliminate the need for cloning DNA, thus reducing the bias often associated with this step. There are some initial improvements in the Aspergillus diversity assessments by NGSTs, which speedup the sequencing and also save time." @default.
- W2511852814 created "2016-09-16" @default.
- W2511852814 creator A5000381401 @default.
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- W2511852814 date "2016-01-01" @default.
- W2511852814 modified "2023-09-27" @default.
- W2511852814 title "Understanding the Diversity of Aspergillus by Next-Generation Sequencing" @default.
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