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• W2512285306 abstract "The assembly of plasmonic metal nanoparticles into hot spot surface-enhanced Raman scattering (SERS) nanocluster probes is a powerful, yet challenging approach for ultrasensitive biosensing. Scaffolding strategies based on self-complementary peptides and proteins are of increasing interest for these assemblies, but the electronic and the photonic properties of such hybrid nanoclusters remain difficult to predict and optimize. Here, split-green fluorescence protein (sGFP) fragments are used as molecular glue and the GFP chromophore is used as a Raman reporter to assemble a variety of gold nanoparticle (AuNP) clusters and explore their plasmonic properties by numerical modeling. It is shown that GFP seeding of plasmonic nanogaps in AuNP/GFP hybrid nanoclusters increases near-field dipolar couplings between AuNPs and provides SERS enhancement factors above 108. Among the different nanoclusters studied, AuNP/GFP chains allow near-infrared SERS detection of the GFP chromophore imidazolinone/exocyclic CC vibrational mode with theoretical enhancement factors of 108–109. For larger AuNP/GFP assemblies, the presence of non-GFP seeded nanogaps between tightly packed nanoparticles reduces near-field enhancements at Raman active hot spots, indicating that excessive clustering can decrease SERS amplifications. This study provides rationales to optimize the controlled assembly of hot spot SERS nanoprobes for remote biosensing using Raman reporters that act as molecular glue between plasmonic nanoparticles." @default.
• W2512285306 created "2016-09-16" @default.
• W2512285306 creator A5054414438 @default.
• W2512285306 creator A5060214768 @default.
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• W2512285306 date "2016-09-08" @default.
• W2512285306 modified "2023-10-14" @default.
• W2512285306 title "Split‐GFP: SERS Enhancers in Plasmonic Nanocluster Probes" @default.
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• W2512285306 doi "https://doi.org/10.1002/smll.201601631" @default.
• W2512285306 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27608276" @default.
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