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• W2512290736 startingPage "9641" @default.
• W2512290736 abstract "Increased pentose phosphate pathway flux, relative to total substrate uptake flux, is shown to enhance succinic acid (SA) yields under continuous, non-growth conditions of Actinobacillus succinogenes biofilms. Separate fermentations of glucose and xylose were conducted in a custom, continuous biofilm reactor at four different dilution rates. Glucose-6-phosphate dehydrogenase assays were performed on cell extracts derived from in situ removal of biofilm at each steady state. The results of the assays were coupled to a kinetic model that revealed an increase in oxidative pentose phosphate pathway (OPPP) flux relative to total substrate flux with increasing SA titre, for both substrates. Furthermore, applying metabolite concentration data to metabolic flux models that include the OPPP revealed similar flux relationships to those observed in the experimental kinetic analysis. A relative increase in OPPP flux produces additional reduction power that enables increased flux through the reductive branch of the TCA cycle, leading to increased SA yields, reduced by-product formation and complete closure of the overall redox balance." @default.
• W2512290736 created "2016-09-16" @default.
• W2512290736 creator A5057867037 @default.
• W2512290736 creator A5073988647 @default.
• W2512290736 date "2016-09-09" @default.
• W2512290736 modified "2023-09-24" @default.
• W2512290736 title "The pentose phosphate pathway leads to enhanced succinic acid flux in biofilms of wild-type Actinobacillus succinogenes" @default.
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• W2512290736 doi "https://doi.org/10.1007/s00253-016-7763-6" @default.
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• W2512290736 hasPublicationYear "2016" @default.
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