Matches in SemOpenAlex for { <https://semopenalex.org/work/W2512554017> ?p ?o ?g. }
- W2512554017 endingPage "4437" @default.
- W2512554017 startingPage "4424" @default.
- W2512554017 abstract "The type VI secretion system (T6SS) is a multiprotein complex that catalyses toxin secretion through the bacterial cell envelope of various Gram-negative bacteria including important human pathogens. This machine uses a bacteriophage-like contractile tail to puncture the prey cell and inject harmful toxins. The T6SS tail comprises an inner tube capped by the cell-puncturing spike and wrapped by the contractile sheath. This structure is built on an assembly platform, the baseplate, which is anchored to the bacterial cell envelope by the TssJLM membrane complex (MC). This MC serves as both a tail docking station and a channel for the passage of the inner tube. The TssM transmembrane protein is a key component of the MC as it connects the inner and outer membranes. In this study, we define the TssM topology, highlighting a large but poorly studied 35-kDa cytoplasmic domain, TssMCyto, located between two transmembrane segments. Protein–protein interaction assays further show that TssMCyto oligomerises and makes contacts with several baseplate components. Using computer predictions, we delineate two subdomains in TssMCyto, including a nucleotide triphosphatase (NTPase) domain, followed by a 110-aa extension. Finally, site-directed mutagenesis coupled to functional assays reveals the contribution of these subdomains and conserved motifs to the interaction with T6SS partners and to the function of the secretion apparatus." @default.
- W2512554017 created "2016-09-16" @default.
- W2512554017 creator A5000220498 @default.
- W2512554017 creator A5018842385 @default.
- W2512554017 creator A5039472552 @default.
- W2512554017 creator A5057279261 @default.
- W2512554017 creator A5065542253 @default.
- W2512554017 date "2016-11-01" @default.
- W2512554017 modified "2023-10-14" @default.
- W2512554017 title "Molecular Dissection of the Interface between the Type VI Secretion TssM Cytoplasmic Domain and the TssG Baseplate Component" @default.
- W2512554017 cites W1526754730 @default.
- W2512554017 cites W1537441966 @default.
- W2512554017 cites W1826521204 @default.
- W2512554017 cites W1899329792 @default.
- W2512554017 cites W1907385644 @default.
- W2512554017 cites W1919814215 @default.
- W2512554017 cites W1936440524 @default.
- W2512554017 cites W1975414190 @default.
- W2512554017 cites W1976555793 @default.
- W2512554017 cites W1979844734 @default.
- W2512554017 cites W1980407081 @default.
- W2512554017 cites W1990621030 @default.
- W2512554017 cites W1995590346 @default.
- W2512554017 cites W2004313328 @default.
- W2512554017 cites W2004798786 @default.
- W2512554017 cites W2005856399 @default.
- W2512554017 cites W2009335881 @default.
- W2512554017 cites W2012109285 @default.
- W2512554017 cites W2023506390 @default.
- W2512554017 cites W2023784732 @default.
- W2512554017 cites W2026487613 @default.
- W2512554017 cites W2038186289 @default.
- W2512554017 cites W2040721088 @default.
- W2512554017 cites W2047670590 @default.
- W2512554017 cites W2053493806 @default.
- W2512554017 cites W2061796105 @default.
- W2512554017 cites W2065235521 @default.
- W2512554017 cites W2068849781 @default.
- W2512554017 cites W2070071915 @default.
- W2512554017 cites W2071464549 @default.
- W2512554017 cites W2082667898 @default.
- W2512554017 cites W2088040325 @default.
- W2512554017 cites W2090535305 @default.
- W2512554017 cites W2094643746 @default.
- W2512554017 cites W2095725095 @default.
- W2512554017 cites W2099816214 @default.
- W2512554017 cites W2101124075 @default.
- W2512554017 cites W2107731942 @default.
- W2512554017 cites W2110770898 @default.
- W2512554017 cites W2111198831 @default.
- W2512554017 cites W2116137883 @default.
- W2512554017 cites W2117484859 @default.
- W2512554017 cites W2122095711 @default.
- W2512554017 cites W2123239729 @default.
- W2512554017 cites W2125957234 @default.
- W2512554017 cites W2132629607 @default.
- W2512554017 cites W2132957039 @default.
- W2512554017 cites W2133394828 @default.
- W2512554017 cites W2145859310 @default.
- W2512554017 cites W2147715428 @default.
- W2512554017 cites W2149525061 @default.
- W2512554017 cites W2150158340 @default.
- W2512554017 cites W2152627116 @default.
- W2512554017 cites W2152770371 @default.
- W2512554017 cites W2155933274 @default.
- W2512554017 cites W2158906453 @default.
- W2512554017 cites W2166783616 @default.
- W2512554017 cites W2168383728 @default.
- W2512554017 cites W2168737114 @default.
- W2512554017 cites W2217161021 @default.
- W2512554017 cites W2219826623 @default.
- W2512554017 cites W2280431758 @default.
- W2512554017 cites W2406492630 @default.
- W2512554017 cites W2415879631 @default.
- W2512554017 cites W2419295916 @default.
- W2512554017 cites W2514438374 @default.
- W2512554017 doi "https://doi.org/10.1016/j.jmb.2016.08.032" @default.
- W2512554017 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27600411" @default.
- W2512554017 hasPublicationYear "2016" @default.
- W2512554017 type Work @default.
- W2512554017 sameAs 2512554017 @default.
- W2512554017 citedByCount "36" @default.
- W2512554017 countsByYear W25125540172016 @default.
- W2512554017 countsByYear W25125540172017 @default.
- W2512554017 countsByYear W25125540172018 @default.
- W2512554017 countsByYear W25125540172019 @default.
- W2512554017 countsByYear W25125540172020 @default.
- W2512554017 countsByYear W25125540172022 @default.
- W2512554017 countsByYear W25125540172023 @default.
- W2512554017 crossrefType "journal-article" @default.
- W2512554017 hasAuthorship W2512554017A5000220498 @default.
- W2512554017 hasAuthorship W2512554017A5018842385 @default.
- W2512554017 hasAuthorship W2512554017A5039472552 @default.
- W2512554017 hasAuthorship W2512554017A5057279261 @default.
- W2512554017 hasAuthorship W2512554017A5065542253 @default.
- W2512554017 hasBestOaLocation W25125540172 @default.
- W2512554017 hasConcept C104317684 @default.
- W2512554017 hasConcept C11804247 @default.