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• W2512896796 abstract "In recent years, there have been some reports suggesting that embryos are evaluated as no pronuclei and two polar bodies (0PN2PB) using conventional microscopic observation but the embryos observed by time-lapse shows that these 2PN break down before the time when we normally observe (2PN-BD). However there are very few articles discussing the process and impact from such PN breakdown. Here, we report whether we can reduce the risk for incorrect observation of embryos following insemination by using time-lapse technology. From July 2014 to September 2014, 448 cycles with 2338 oocytes were cultured for 6h following conventional IVF (cIVF) or ICSI and observed at 20h after insemination in Embryo Scope (Vitrolife) then we cultured the embryos into moist incubator until Day5. We used Veeck’s criteria and Gardner’s criteria for embryo evaluation. Treatments used TESE-ICSI and rescue-ICSI and calcium ionophore were excluded. We calculated the ratio for which embryos were evaluated as 0PN2PB by conventional microscopic observation but showed 2PN by time-lapse observation. We also studied the cleavage rate, Day3 good quality embryo rate, Day5 blastocyst rate, and Day5 good quality blastocyst rate of these embryos. Furthermore, we analyzed the time from PN appearance to its breakdown. All statistical analyses were performed using chi squared analysis. Among 281 embryos which were evaluated as 0PN2PB using conventional microscopic observation, 141 embryos were evaluated as 2PN by time-lapse observation. The cIVF group has 2PN-BD embryos of 33.6% (38/113) and the ICSI group has the embryos of 61.3% (103/168). The 2PN-BD embryos show same development compared 2PN embryos in cIVF group. However, in the ICSI group, Day3 good quality rate, Day5 blastocyst rate and Day5 good quality blastocyst rate were significantly higher in the 2PN-BD group (P< 0.01). For the 1825 embryos which were evaluated as 2PN by time-lapse observation, the time from insemination to 2PN appearance was as follows; 8.6h±0.1h (5.5h-19.4h) for the cIVF group, and 6.9h±0.1h (3.6h-22.3h) for the ICSI group. The embryos which developed into 2PN later than 15 hours following insemination did not grow into good quality blastocysts. Finally, the time of 2PN-BD was 15.9h (ICSI) and 17.2h (cIVF) following insemination at earliest. It is very difficult to get the information of correct fertilization without using time-lapse system, because there are some embryos whose pronuclei break down early. Time-lapse technology can also help us improve workflow in the lab since actual observation does not need to be done at a specific time." @default.
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• W2512896796 date "2016-09-01" @default.
• W2512896796 modified "2023-10-15" @default.
• W2512896796 title "Time-lapse observation can help improve workflow and ensure the correct observation of fertilized embryos" @default.
• W2512896796 doi "https://doi.org/10.1016/j.fertnstert.2016.07.996" @default.
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