FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2513222912> ?p ?o ?g. }

• W2513222912 abstract "Abstract Abstract 942 Background: Emerging evidence suggests that microRNAs (miRNAs) are critical in cancer and adult leukemia by functioning as tumor suppressors and/or oncogenes. Zhang et al identified 32 pediatric acute myeloid leukemia (AML)-specific miRNA patterns by analysis of bone marrow (BM) samples (1). They also established potential miRNAs as biomarkers for predicting CNS-relapse in pediatric acute lymphocytic leukemia (ALL). Altered miRNA expression disrupts normal hematopoiesis and might play a role in niche-induced oncogenesis. Dysfunction of mesenchymal stromal cells induces formation of myeloid sarcomas that infiltrate in the surrounding tissues (2). Previously, we described that mesenchymal stem cells (MSC) of the BM microenvironment participate in leukemic stem cell regulation in an in vivo model of the childhood AML stem cell niche(3). These human MSC niches, created in ectopic bioengineered 3D scaffolds, supported leukemogenesis in NOD/SCID mice. Pediatric AML engrafted at 1 month in the MSC-coated scaffolds in the mice and was retained in the niche up to 4 months, after which distant seeding to murine BM, liver and spleen occurred. The bioengineered niche created a sanctuary for quiescent leukemia cells and at 4 months the AML cells exited the niche and spread hematogenously, mimicking leukemia relapse. Analysis of miRNA patterns in our leukemia niche model could provide novel directions for individual risk-adapted therapy in childhood leukemia. Objective: To analyze miRNA expression patterns of pediatric AML after exposure to the niche microenvironment at different time points. Design and Method: miRNAs were obtained from primary CD34+ selected AML cells at (d0) Day0= no niche exposure, (1Mo) 1 month =niche engraftment, (4Mo) 4 months=hematogenous spread with leukemic exit from the niche. miRNAs were isolated from single cell suspensions with the mirVana miRNA isolation kit (Ambion) and analyzed on an Ilumina MicroRNA Expression Profiling single Beadchip (#RNA probes = 1145). Results: 498/1145 miRNAs expression profiles were selected with a detection p value < 0.00001. Out of 498 miRNAs expressed in the leukemic niche model, 23 were previously described as AML-specific miRNAs (2). 10/23 miRNAs were significantly upregulated and 13/23 were downregulated. Pediatric AML-specific miRNAs – miR100 and miR125b had high expression profile at baseline, but were down regulated upon contact with the niche. AML miR195 and miR193a had low expression at baseline, but miR195 was upregulated on engraftment while miR193a only upregulated at the time of hematogenous spread (niche exit). CNS-relapse in ALL might represent a physiological mechanism of leukemic exit of dormant cells from the niche sanctuary. Consistent with this notion, the same expression profile that was found in CNS-relapse in ALL patients (miR198 up – miR551a downregulated) was seen in our model when AML cells became invasive and exited the niche at 4 months. Conclusion: (1) Zhang et al. 2009, PloSOne, 4, p1 (2) Raaijmakers et al. 2010, Nature, 464, p852 (3) Vaiselbuh et al, 2010, Tissue Eng Part C Methods, June 29 (ePub) Disclosures: No relevant conflicts of interest to declare." @default.
• W2513222912 created "2016-09-16" @default.
• W2513222912 creator A5007547414 @default.
• W2513222912 creator A5028026915 @default.
• W2513222912 creator A5044759942 @default.
• W2513222912 creator A5060331249 @default.
• W2513222912 creator A5066232855 @default.
• W2513222912 creator A5082248535 @default.
• W2513222912 date "2010-11-19" @default.
• W2513222912 modified "2023-09-30" @default.
• W2513222912 title "Pediatric Leukemia-Specific miRNA Expression Profiles Induced by the Leukemic Stem Cell Niche" @default.
• W2513222912 doi "https://doi.org/10.1182/blood.v116.21.942.942" @default.
• W2513222912 hasPublicationYear "2010" @default.
• W2513222912 type Work @default.
• W2513222912 sameAs 2513222912 @default.
• W2513222912 citedByCount "0" @default.
• W2513222912 crossrefType "journal-article" @default.
• W2513222912 hasAuthorship W2513222912A5007547414 @default.
• W2513222912 hasAuthorship W2513222912A5028026915 @default.
• W2513222912 hasAuthorship W2513222912A5044759942 @default.
• W2513222912 hasAuthorship W2513222912A5060331249 @default.
• W2513222912 hasAuthorship W2513222912A5066232855 @default.
• W2513222912 hasAuthorship W2513222912A5082248535 @default.
• W2513222912 hasConcept C10205521 @default.
• W2513222912 hasConcept C104317684 @default.
• W2513222912 hasConcept C109159458 @default.
• W2513222912 hasConcept C145059251 @default.
• W2513222912 hasConcept C16930146 @default.
• W2513222912 hasConcept C198826908 @default.
• W2513222912 hasConcept C203014093 @default.
• W2513222912 hasConcept C2778461978 @default.
• W2513222912 hasConcept C2778729363 @default.
• W2513222912 hasConcept C2780007613 @default.
• W2513222912 hasConcept C28328180 @default.
• W2513222912 hasConcept C502942594 @default.
• W2513222912 hasConcept C54355233 @default.
• W2513222912 hasConcept C81885089 @default.
• W2513222912 hasConcept C86803240 @default.
• W2513222912 hasConcept C95444343 @default.
• W2513222912 hasConcept C97037327 @default.
• W2513222912 hasConceptScore W2513222912C10205521 @default.
• W2513222912 hasConceptScore W2513222912C104317684 @default.
• W2513222912 hasConceptScore W2513222912C109159458 @default.
• W2513222912 hasConceptScore W2513222912C145059251 @default.
• W2513222912 hasConceptScore W2513222912C16930146 @default.
• W2513222912 hasConceptScore W2513222912C198826908 @default.
• W2513222912 hasConceptScore W2513222912C203014093 @default.
• W2513222912 hasConceptScore W2513222912C2778461978 @default.
• W2513222912 hasConceptScore W2513222912C2778729363 @default.
• W2513222912 hasConceptScore W2513222912C2780007613 @default.
• W2513222912 hasConceptScore W2513222912C28328180 @default.
• W2513222912 hasConceptScore W2513222912C502942594 @default.
• W2513222912 hasConceptScore W2513222912C54355233 @default.
• W2513222912 hasConceptScore W2513222912C81885089 @default.
• W2513222912 hasConceptScore W2513222912C86803240 @default.
• W2513222912 hasConceptScore W2513222912C95444343 @default.
• W2513222912 hasConceptScore W2513222912C97037327 @default.
• W2513222912 hasLocation W25132229121 @default.
• W2513222912 hasOpenAccess W2513222912 @default.
• W2513222912 hasPrimaryLocation W25132229121 @default.
• W2513222912 hasRelatedWork W1971445586 @default.
• W2513222912 hasRelatedWork W1996545149 @default.
• W2513222912 hasRelatedWork W205262919 @default.
• W2513222912 hasRelatedWork W2068839651 @default.
• W2513222912 hasRelatedWork W2152251220 @default.
• W2513222912 hasRelatedWork W2258643423 @default.
• W2513222912 hasRelatedWork W2419001201 @default.
• W2513222912 hasRelatedWork W2528585563 @default.
• W2513222912 hasRelatedWork W2530940620 @default.
• W2513222912 hasRelatedWork W2531244026 @default.
• W2513222912 hasRelatedWork W2531803230 @default.
• W2513222912 hasRelatedWork W2555030899 @default.
• W2513222912 hasRelatedWork W2575981405 @default.
• W2513222912 hasRelatedWork W2582459711 @default.
• W2513222912 hasRelatedWork W2765259899 @default.
• W2513222912 hasRelatedWork W2785502620 @default.
• W2513222912 hasRelatedWork W2789164439 @default.
• W2513222912 hasRelatedWork W2911548464 @default.
• W2513222912 hasRelatedWork W2963999192 @default.
• W2513222912 hasRelatedWork W3183944997 @default.
• W2513222912 isParatext "false" @default.
• W2513222912 isRetracted "false" @default.
• W2513222912 magId "2513222912" @default.
• W2513222912 workType "article" @default.