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• W2513480708 abstract "DNA methylation is one of the epigenetic mechanisms which have been implicated in cellular differentiation, ageing and disease development. The effect of hypomethylating drug 5-aza-2&acute;deoxycytidine (5-aza dC) on the biosynthetic profile of caudal region chondrocytes from chick sternum was studied in detail. The chondrocytes in culture were treated with varying doses of 5-aza dC for 48h and maintained subsequently without the treatment and harvested at selected time points for analysis of growth and differentiation status. 15&micro;g/ml of 5-aza dC showed optimum Concentration at which there was a significant increase in DNA synthesis and RNA synthesis as per cell basis. There was also a significant increase in total protein synthesis and collagen synthesis as per cell basis at this concentration. This optimal concentration also showed to up regulate the gene expression of Type X collagen and alkaline phosphatase, which are the marker of hypertrophic chondrocyte expression. These results further support the notion that methylation is the major epigenetic factor controlling the differentiation and maturation of chondrocytes." @default.
• W2513480708 created "2016-09-16" @default.
• W2513480708 creator A5009200983 @default.
• W2513480708 date "2016-12-27" @default.
• W2513480708 modified "2023-09-26" @default.
• W2513480708 title "Effects of 5 Aza 2^’ Deoxycytidine on Proliferation and Differentiation of Embryonic Chick Caudal Region Chondrocytes in Culture" @default.
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• W2513480708 doi "https://doi.org/10.20944/preprints201608.0176.v2" @default.
• W2513480708 hasPublicationYear "2016" @default.
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