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- W2513486036 abstract "Abstract We study synthesis of L -phenylserine catalyzed by the enzyme L -threonine aldolase in a slug-flow microfluidic system. Slug-flow arrangement allows for the continuous refilling of sparingly soluble substrate (benzaldehyde) into an aqueous reaction mixture. We identified suitable composition of an organic phase to provide stable slug-flow in a wide range of operational parameters. Solvent screening revealed that tert-butyl methyl ether (TBME) as the organic solvent provides the most friendly environment for L -phenylserine synthesis due to a low degree of enzyme deactivation and high benzaldehyde concentration in the reaction mixture. The effects of substrate concentrations, enzyme concentration, and dimethylsulfoxide (DMSO) concentration on the L -phenylserine concentration in the product stream were examined and proper reaction conditions were identified. Experimental results on the L -phenylserine diastereoselectivity demonstrate that the amount of syn -conformation of L -phenylserine increases with the reaction time. High syn - to anti - concentration ratio is achieved with 2-methyltetrahydrofuran (MeTHF) solvent in a system with long residence time and, finally, low syn- to anti - concentration ratio is provided by toluene environment and short residence time." @default.
- W2513486036 created "2016-09-16" @default.
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- W2513486036 date "2017-09-01" @default.
- W2513486036 modified "2023-10-17" @default.
- W2513486036 title "Aldolase catalyzed L-phenylserine synthesis in a slug-flow microfluidic system – Performance and diastereoselectivity studies" @default.
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- W2513486036 doi "https://doi.org/10.1016/j.ces.2016.08.033" @default.
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