FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2513668231> ?p ?o ?g. }

• W2513668231 endingPage "903" @default.
• W2513668231 startingPage "897" @default.
• W2513668231 abstract "Summary Polyphosphate (polyP) binds to fibrin(ogen) and alters fibrin structure, generating a heterogeneous network composed of ‘knots’ interspersed by large pores. Here we show platelet-derived polyP elicits similar structural changes in fibrin and examine the mechanism by which polyP alters fibrin structure. Polymerisation of fibrinogen with thrombin and CaCl2 was studied using spinning disk confocal (SDC) microscopy. PolyP delayed fibrin polymerisation generating shorter protofibrils emanating from a nucleus-type structure. Consistent with this, cascade blue-polyP accumulated in fibrin ‘knots’. Protofibril formation was visualized by atomic force microscopy (AFM) ± polyP. In the presence of polyP abundant monomers of longer length were visualised by AFM, suggesting that polyP binds to monomeric fibrin. Shorter oligomers form in the presence of polyP, consistent with the stunted protofibrils visualised by SDC microscopy. We examined whether these structural changes induced by polyP alter fibrin’s viscoelastic properties by rheometry. PolyP reduced the stiffness (G’) and ability of the fibrin network to deform plastically G”, but to different extents. Consequently, the relative plastic component (loss tangent (G”/G’)) was 61 % higher implying that networks containing polyP are less stiff and more plastic. Local rheological measurements, performed using magnetic tweezers, indicate that the fibrin dense knots are stiffer and more plastic, reflecting the heterogeneity of the network. Our data show that polyP impedes fibrin polymerisation, stunting protofibril growth producing ‘knotted’ regions, which are rich in fibrin and polyP. Consequently, the mechanical properties of the fibrin network are altered resulting in clots with overall reduced stiffness and increased ability to deform plastically. Supplementary Material to this article is available online at www.thrombosis-online.com." @default.
• W2513668231 created "2016-09-16" @default.
• W2513668231 creator A5008277035 @default.
• W2513668231 creator A5031527489 @default.
• W2513668231 creator A5048558779 @default.
• W2513668231 creator A5064269852 @default.
• W2513668231 creator A5073299811 @default.
• W2513668231 creator A5078965269 @default.
• W2513668231 creator A5089911552 @default.
• W2513668231 date "2016-01-01" @default.
• W2513668231 modified "2023-10-12" @default.
• W2513668231 title "Polyphosphate delays fibrin polymerisation and alters the mechanical properties of the fibrin network" @default.
• W2513668231 cites W146872388 @default.
• W2513668231 cites W1711208506 @default.
• W2513668231 cites W1816831867 @default.
• W2513668231 cites W1907444416 @default.
• W2513668231 cites W1968468186 @default.
• W2513668231 cites W1984353324 @default.
• W2513668231 cites W1987969307 @default.
• W2513668231 cites W1988873606 @default.
• W2513668231 cites W1994007436 @default.
• W2513668231 cites W1999681205 @default.
• W2513668231 cites W2000502450 @default.
• W2513668231 cites W2002572330 @default.
• W2513668231 cites W2011339820 @default.
• W2513668231 cites W2033458237 @default.
• W2513668231 cites W2035998381 @default.
• W2513668231 cites W2046971159 @default.
• W2513668231 cites W2057007282 @default.
• W2513668231 cites W2057646074 @default.
• W2513668231 cites W2058842531 @default.
• W2513668231 cites W2060792403 @default.
• W2513668231 cites W2062949495 @default.
• W2513668231 cites W2064232485 @default.
• W2513668231 cites W2065880897 @default.
• W2513668231 cites W2079132877 @default.
• W2513668231 cites W2082279654 @default.
• W2513668231 cites W2083884362 @default.
• W2513668231 cites W2084124265 @default.
• W2513668231 cites W2087458203 @default.
• W2513668231 cites W2092013656 @default.
• W2513668231 cites W2092707717 @default.
• W2513668231 cites W2095486848 @default.
• W2513668231 cites W2100876879 @default.
• W2513668231 cites W2113351822 @default.
• W2513668231 cites W2116209993 @default.
• W2513668231 cites W2119581653 @default.
• W2513668231 cites W2144537409 @default.
• W2513668231 cites W2265447718 @default.
• W2513668231 cites W2317045510 @default.
• W2513668231 cites W2408538721 @default.
• W2513668231 doi "https://doi.org/10.1160/th16-01-0062" @default.
• W2513668231 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5228039" @default.
• W2513668231 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27610454" @default.
• W2513668231 hasPublicationYear "2016" @default.
• W2513668231 type Work @default.
• W2513668231 sameAs 2513668231 @default.
• W2513668231 citedByCount "17" @default.
• W2513668231 countsByYear W25136682312017 @default.
• W2513668231 countsByYear W25136682312018 @default.
• W2513668231 countsByYear W25136682312019 @default.
• W2513668231 countsByYear W25136682312020 @default.
• W2513668231 countsByYear W25136682312021 @default.
• W2513668231 countsByYear W25136682312022 @default.
• W2513668231 countsByYear W25136682312023 @default.
• W2513668231 crossrefType "journal-article" @default.
• W2513668231 hasAuthorship W2513668231A5008277035 @default.
• W2513668231 hasAuthorship W2513668231A5031527489 @default.
• W2513668231 hasAuthorship W2513668231A5048558779 @default.
• W2513668231 hasAuthorship W2513668231A5064269852 @default.
• W2513668231 hasAuthorship W2513668231A5073299811 @default.
• W2513668231 hasAuthorship W2513668231A5078965269 @default.
• W2513668231 hasAuthorship W2513668231A5089911552 @default.
• W2513668231 hasBestOaLocation W25136682311 @default.
• W2513668231 hasConcept C105702510 @default.
• W2513668231 hasConcept C12554922 @default.
• W2513668231 hasConcept C159985019 @default.
• W2513668231 hasConcept C185592680 @default.
• W2513668231 hasConcept C191172559 @default.
• W2513668231 hasConcept C192562407 @default.
• W2513668231 hasConcept C200990466 @default.
• W2513668231 hasConcept C203014093 @default.
• W2513668231 hasConcept C27523624 @default.
• W2513668231 hasConcept C2777292125 @default.
• W2513668231 hasConcept C2779036427 @default.
• W2513668231 hasConcept C44228677 @default.
• W2513668231 hasConcept C521977710 @default.
• W2513668231 hasConcept C54173615 @default.
• W2513668231 hasConcept C55493867 @default.
• W2513668231 hasConcept C86803240 @default.
• W2513668231 hasConcept C89560881 @default.
• W2513668231 hasConceptScore W2513668231C105702510 @default.
• W2513668231 hasConceptScore W2513668231C12554922 @default.
• W2513668231 hasConceptScore W2513668231C159985019 @default.
• W2513668231 hasConceptScore W2513668231C185592680 @default.
• W2513668231 hasConceptScore W2513668231C191172559 @default.
• W2513668231 hasConceptScore W2513668231C192562407 @default.
• W2513668231 hasConceptScore W2513668231C200990466 @default.

• next