Matches in SemOpenAlex for { <https://semopenalex.org/work/W2513746499> ?p ?o ?g. }
Showing items 1 to 65 of
65
with 100 items per page.
- W2513746499 endingPage "e233" @default.
- W2513746499 startingPage "e233" @default.
- W2513746499 abstract "Background: Episodic ataxia type 1 (EA1) is an autosomal dominant disorder characterized by paroxysmal cerebellar incoordination and interictal myokymia (Rajakulendran et al., 2007). It is caused by dominant negative mutations of KCNA1, which encodes the presynaptic and axonal potassium channel subunit Kv1.1. EA1 mutations increase neuronal excitability and neurotransmitter release in neuronal cultures (Heeroma et al., 2009). Although this could be explained by impaired action potential repolarization leading to an increase in presynaptic calcium influx, the action potential duration measured at the cell body is unaffected. Objective: Does EA1 prolong the action potential repolarization phase at presynaptic terminals thus causing increased neurotransmitter release? Method: We used Scanning Ion Conductance Microscopy to acquire super-resolution images of very small synaptic boutons (approximately 1 micrometer diameter) in mouse hippocampal neuronal cultures and gain direct electrophysiological recording from these boutons (Novak et al, 2013). We compared wild type, KCNA1a-/- knock-out and KCNA1a(V408A/+) knock-in mice (Herson et al., 2003). Action potentials were either elicited by injecting depolarizing current at the bouton or via a second pipette at the soma. Results: Presynaptic action potential half width was larger in KCNA1a-/- and KCNA1a(V408A/+) compared to their wild type litter mates, both when directly elicited at the bouton and when elicited by depolarising the cell body. A prolonged presynaptic action potential in EA1 may be responsible for increased neurotransmitter release. Because Kv1.1 is abundantly expressed in cerebellar basket cells, this may lead to excessive inhibition of cerebellar Purkinje cells and ataxia." @default.
- W2513746499 created "2016-09-16" @default.
- W2513746499 creator A5033570060 @default.
- W2513746499 creator A5039202630 @default.
- W2513746499 creator A5063777312 @default.
- W2513746499 date "2015-10-01" @default.
- W2513746499 modified "2023-09-25" @default.
- W2513746499 title "Study on presynaptic action potential waveform in hippocampal neuronal culture models of episodic ataxia type 1 using scanning ion conductance microscopy" @default.
- W2513746499 doi "https://doi.org/10.1016/j.jns.2015.08.800" @default.
- W2513746499 hasPublicationYear "2015" @default.
- W2513746499 type Work @default.
- W2513746499 sameAs 2513746499 @default.
- W2513746499 citedByCount "0" @default.
- W2513746499 crossrefType "journal-article" @default.
- W2513746499 hasAuthorship W2513746499A5033570060 @default.
- W2513746499 hasAuthorship W2513746499A5039202630 @default.
- W2513746499 hasAuthorship W2513746499A5063777312 @default.
- W2513746499 hasConcept C12554922 @default.
- W2513746499 hasConcept C148762608 @default.
- W2513746499 hasConcept C169760540 @default.
- W2513746499 hasConcept C170493617 @default.
- W2513746499 hasConcept C185263204 @default.
- W2513746499 hasConcept C185592680 @default.
- W2513746499 hasConcept C2776219046 @default.
- W2513746499 hasConcept C2779652256 @default.
- W2513746499 hasConcept C4141045 @default.
- W2513746499 hasConcept C50254741 @default.
- W2513746499 hasConcept C529278444 @default.
- W2513746499 hasConcept C55493867 @default.
- W2513746499 hasConcept C86803240 @default.
- W2513746499 hasConcept C93872552 @default.
- W2513746499 hasConceptScore W2513746499C12554922 @default.
- W2513746499 hasConceptScore W2513746499C148762608 @default.
- W2513746499 hasConceptScore W2513746499C169760540 @default.
- W2513746499 hasConceptScore W2513746499C170493617 @default.
- W2513746499 hasConceptScore W2513746499C185263204 @default.
- W2513746499 hasConceptScore W2513746499C185592680 @default.
- W2513746499 hasConceptScore W2513746499C2776219046 @default.
- W2513746499 hasConceptScore W2513746499C2779652256 @default.
- W2513746499 hasConceptScore W2513746499C4141045 @default.
- W2513746499 hasConceptScore W2513746499C50254741 @default.
- W2513746499 hasConceptScore W2513746499C529278444 @default.
- W2513746499 hasConceptScore W2513746499C55493867 @default.
- W2513746499 hasConceptScore W2513746499C86803240 @default.
- W2513746499 hasConceptScore W2513746499C93872552 @default.
- W2513746499 hasLocation W25137464991 @default.
- W2513746499 hasOpenAccess W2513746499 @default.
- W2513746499 hasPrimaryLocation W25137464991 @default.
- W2513746499 hasRelatedWork W111279135 @default.
- W2513746499 hasRelatedWork W1825111584 @default.
- W2513746499 hasRelatedWork W2033922711 @default.
- W2513746499 hasRelatedWork W2078408324 @default.
- W2513746499 hasRelatedWork W2094699087 @default.
- W2513746499 hasRelatedWork W2140488599 @default.
- W2513746499 hasRelatedWork W2148347482 @default.
- W2513746499 hasRelatedWork W2400752963 @default.
- W2513746499 hasRelatedWork W3000026933 @default.
- W2513746499 hasRelatedWork W3005239728 @default.
- W2513746499 hasVolume "357" @default.
- W2513746499 isParatext "false" @default.
- W2513746499 isRetracted "false" @default.
- W2513746499 magId "2513746499" @default.
- W2513746499 workType "article" @default.