FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2513860348> ?p ?o ?g. }

• W2513860348 endingPage "108" @default.
• W2513860348 startingPage "103" @default.
• W2513860348 abstract "Sub-optimal recovery of bacterial DNA from whole blood samples can limit the sensitivity of molecular assays to detect pathogenic bacteria. We compared 3 different pre-lysis protocols (none, mechanical pre-lysis and achromopeptidase pre-lysis) and 5 commercially available DNA extraction platforms for direct detection of Group B Streptococcus (GBS) in spiked whole blood samples, without enrichment culture. DNA was extracted using the QIAamp Blood Mini kit (Qiagen), UCP Pathogen Mini kit (Qiagen), QuickGene DNA Whole Blood kit S (Fuji), Speed Xtract Nucleic Acid Kit 200 (Qiagen) and MagNA Pure Compact Nucleic Acid Isolation Kit I (Roche Diagnostics Corp). Mechanical pre-lysis increased yields of bacterial genomic DNA by 51.3 fold (95% confidence interval; 31.6-85.1, p<0.001) and pre-lysis with achromopeptidase by 6.1 fold (95% CI; 4.2-8.9, p<0.001), compared with no pre-lysis. Differences in yield due to pre-lysis were 2-3 fold larger than differences in yield between extraction methods. Including a pre-lysis step can improve the limits of detection of GBS using PCR or other molecular methods without need for culture." @default.
• W2513860348 created "2016-09-16" @default.
• W2513860348 creator A5063860760 @default.
• W2513860348 creator A5071678055 @default.
• W2513860348 creator A5087398782 @default.
• W2513860348 creator A5087865591 @default.
• W2513860348 creator A5088863796 @default.
• W2513860348 creator A5089083023 @default.
• W2513860348 date "2016-10-01" @default.
• W2513860348 modified "2023-10-18" @default.
• W2513860348 title "A comparison of different pre-lysis methods and extraction kits for recovery of Streptococcus agalacticae (Lancefield group B Streptococcus) DNA from whole blood" @default.
• W2513860348 cites W1849444937 @default.
• W2513860348 cites W1864509826 @default.
• W2513860348 cites W1958790533 @default.
• W2513860348 cites W1966939914 @default.
• W2513860348 cites W2023088101 @default.
• W2513860348 cites W2024630309 @default.
• W2513860348 cites W2027546410 @default.
• W2513860348 cites W2054599821 @default.
• W2513860348 cites W2065111414 @default.
• W2513860348 cites W2079897609 @default.
• W2513860348 cites W2088002866 @default.
• W2513860348 cites W2095989309 @default.
• W2513860348 cites W2096713261 @default.
• W2513860348 cites W2126914905 @default.
• W2513860348 cites W2136755398 @default.
• W2513860348 cites W2139183939 @default.
• W2513860348 cites W2147268130 @default.
• W2513860348 cites W2152937580 @default.
• W2513860348 cites W2160848371 @default.
• W2513860348 cites W2169538646 @default.
• W2513860348 cites W2486646539 @default.
• W2513860348 doi "https://doi.org/10.1016/j.mimet.2016.08.016" @default.
• W2513860348 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27546716" @default.
• W2513860348 hasPublicationYear "2016" @default.
• W2513860348 type Work @default.
• W2513860348 sameAs 2513860348 @default.
• W2513860348 citedByCount "10" @default.
• W2513860348 countsByYear W25138603482017 @default.
• W2513860348 countsByYear W25138603482018 @default.
• W2513860348 countsByYear W25138603482020 @default.
• W2513860348 countsByYear W25138603482021 @default.
• W2513860348 countsByYear W25138603482022 @default.
• W2513860348 crossrefType "journal-article" @default.
• W2513860348 hasAuthorship W2513860348A5063860760 @default.
• W2513860348 hasAuthorship W2513860348A5071678055 @default.
• W2513860348 hasAuthorship W2513860348A5087398782 @default.
• W2513860348 hasAuthorship W2513860348A5087865591 @default.
• W2513860348 hasAuthorship W2513860348A5088863796 @default.
• W2513860348 hasAuthorship W2513860348A5089083023 @default.
• W2513860348 hasBestOaLocation W25138603482 @default.
• W2513860348 hasConcept C104317684 @default.
• W2513860348 hasConcept C153911025 @default.
• W2513860348 hasConcept C1621761 @default.
• W2513860348 hasConcept C17757408 @default.
• W2513860348 hasConcept C203014093 @default.
• W2513860348 hasConcept C24107716 @default.
• W2513860348 hasConcept C49105822 @default.
• W2513860348 hasConcept C523546767 @default.
• W2513860348 hasConcept C54355233 @default.
• W2513860348 hasConcept C552990157 @default.
• W2513860348 hasConcept C55493867 @default.
• W2513860348 hasConcept C57409179 @default.
• W2513860348 hasConcept C78063203 @default.
• W2513860348 hasConcept C86803240 @default.
• W2513860348 hasConcept C89423630 @default.
• W2513860348 hasConceptScore W2513860348C104317684 @default.
• W2513860348 hasConceptScore W2513860348C153911025 @default.
• W2513860348 hasConceptScore W2513860348C1621761 @default.
• W2513860348 hasConceptScore W2513860348C17757408 @default.
• W2513860348 hasConceptScore W2513860348C203014093 @default.
• W2513860348 hasConceptScore W2513860348C24107716 @default.
• W2513860348 hasConceptScore W2513860348C49105822 @default.
• W2513860348 hasConceptScore W2513860348C523546767 @default.
• W2513860348 hasConceptScore W2513860348C54355233 @default.
• W2513860348 hasConceptScore W2513860348C552990157 @default.
• W2513860348 hasConceptScore W2513860348C55493867 @default.
• W2513860348 hasConceptScore W2513860348C57409179 @default.
• W2513860348 hasConceptScore W2513860348C78063203 @default.
• W2513860348 hasConceptScore W2513860348C86803240 @default.
• W2513860348 hasConceptScore W2513860348C89423630 @default.
• W2513860348 hasFunder F4320320357 @default.
• W2513860348 hasLocation W25138603481 @default.
• W2513860348 hasLocation W25138603482 @default.
• W2513860348 hasLocation W25138603483 @default.
• W2513860348 hasOpenAccess W2513860348 @default.
• W2513860348 hasPrimaryLocation W25138603481 @default.
• W2513860348 hasRelatedWork W1811554286 @default.
• W2513860348 hasRelatedWork W1966014687 @default.
• W2513860348 hasRelatedWork W1968755513 @default.
• W2513860348 hasRelatedWork W2006124126 @default.
• W2513860348 hasRelatedWork W2088096907 @default.
• W2513860348 hasRelatedWork W2351873555 @default.
• W2513860348 hasRelatedWork W2358788337 @default.
• W2513860348 hasRelatedWork W2382592496 @default.
• W2513860348 hasRelatedWork W2908235239 @default.
• W2513860348 hasRelatedWork W3210389753 @default.
• W2513860348 hasVolume "129" @default.

• next