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- W2514059600 abstract "The gene 4 protein of bacteriophage T7 recog- nizes specific sequences on single-stranded DNA and then cata- lyzes the synthesis of tetraribonucleotide primers complementary to the template. With 4X174 DNA as a template, the gene 4 pro- tein enables T7 DNA polymerase (deoxynucleosidetriphos- phate:DNA deoxynucleotidyltransferase, EC 2.7.7.7) to initiate DNA synthesis at 13 major sites. DNA sequence analysis of the 5' termini of the newly synthesized DNA shows the predominant rec- ognition sequences for the gene 4 protein to be 3'-C-T-G-G-G-5' or 3'-C-T-G-G-T-5'; the products of synthesis at these sites are RNA primers having the sequences pppA-C-C-C or pppA-C-C-A. The gene 4 protein can also synthesize primers at the sequences 3'-C-T-G-G-A-5' and 3'-C-T-G-T-N-5', although these sites are used less than 10% as frequently as the predominant sites. Com- parison of the utilization of primer sites suggests that the gene 4 protein binds randomly to single-stranded DNA and then translo- cates along the DNA in a unidirectional 5'-to-3' direction with re- gard to the DNA strand in search of recognition sequences. Models are presented for the role of the gene 4 protein in the initiation of lagging-strand synthesis and in the initiation of DNA replication at the origin." @default.
- W2514059600 created "2016-09-16" @default.
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- W2514059600 date "2016-01-01" @default.
- W2514059600 modified "2023-09-27" @default.
- W2514059600 title "Template recognition sequence for I protein of bacteriophage T7* (DNA initiation/primase/lagging-strand synthesis/Okazaki fragments/" @default.
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