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• W2514134090 endingPage "4981" @default.
• W2514134090 startingPage "4971" @default.
• W2514134090 abstract "The first step in removing cholesterol from a cell is the ATP-binding cassette transporter 1 (ABCA1)-driven transfer of cholesterol to lipid-free or lipid-poor apolipoprotein A-I (apoA-I), which yields cholesterol-rich nascent high-density lipoprotein (nHDL) that then matures in plasma to spherical, cholesteryl ester-rich HDL. However, lipid-free apoA-I has a three-dimensional (3D) conformation that is significantly different from that of lipidated apoA-I on nHDL. By comparing the lipid-free apoA-I 3D conformation of apoA-I to that of 9–14 nm diameter nHDL, we formulated the hypothetical helical domain transitions that might drive particle formation. To test the hypothesis, ten apoA-I mutants were prepared that contained two strategically placed cysteines several of which could form intramolecular disulfide bonds and others that could not form these bonds. Mass spectrometry was used to identify amino acid sequence and intramolecular disulfide bond formation. Recombinant HDL (rHDL) formation was assessed with this group of apoA-I mutants. ABCA1-driven nHDL formation was measured in four mutants and wild-type apoA-I. The mutants contained cysteine substitutions in one of three regions: the N-terminus, amino acids 34 and 55 (E34C to S55C), central domain amino acids 104 and 162 (F104C to H162C), and the C-terminus, amino acids 200 and 233 (L200C to L233C). Mutants were studied in the locked form, with an intramolecular disulfide bond present, or unlocked form, with the cysteine thiol blocked by alkylation. Only small amounts of rHDL or nHDL were formed upon locking the central domain. We conclude that both the N- and C-terminal ends assist in the initial steps in lipid acquisition, but that opening of the central domain was essential for particle formation." @default.
• W2514134090 created "2016-09-16" @default.
• W2514134090 creator A5005935544 @default.
• W2514134090 creator A5012217431 @default.
• W2514134090 creator A5058164261 @default.
• W2514134090 creator A5084285774 @default.
• W2514134090 date "2016-08-23" @default.
• W2514134090 modified "2023-10-14" @default.
• W2514134090 title "High-Density Lipoprotein Biogenesis: Defining the Domains Involved in Human Apolipoprotein A-I Lipidation" @default.
• W2514134090 cites W1509309208 @default.
• W2514134090 cites W1524712279 @default.
• W2514134090 cites W1547902023 @default.
• W2514134090 cites W1580807016 @default.
• W2514134090 cites W1598788587 @default.
• W2514134090 cites W1775749144 @default.
• W2514134090 cites W1947043095 @default.
• W2514134090 cites W1965277932 @default.
• W2514134090 cites W1966129095 @default.
• W2514134090 cites W1966472921 @default.
• W2514134090 cites W1970652477 @default.
• W2514134090 cites W1973858093 @default.
• W2514134090 cites W1976877788 @default.
• W2514134090 cites W1978921170 @default.
• W2514134090 cites W1980064899 @default.
• W2514134090 cites W1983735773 @default.
• W2514134090 cites W1986524045 @default.
• W2514134090 cites W1987271370 @default.
• W2514134090 cites W1989249306 @default.
• W2514134090 cites W1993012149 @default.
• W2514134090 cites W1994618293 @default.
• W2514134090 cites W1999498121 @default.
• W2514134090 cites W2004353149 @default.
• W2514134090 cites W2009979715 @default.
• W2514134090 cites W2012288008 @default.
• W2514134090 cites W2012534919 @default.
• W2514134090 cites W2014409886 @default.
• W2514134090 cites W2020331655 @default.
• W2514134090 cites W2022619537 @default.
• W2514134090 cites W2026507477 @default.
• W2514134090 cites W2027447962 @default.
• W2514134090 cites W2027927035 @default.
• W2514134090 cites W2029902514 @default.
• W2514134090 cites W2032055079 @default.
• W2514134090 cites W2041136477 @default.
• W2514134090 cites W2041295337 @default.
• W2514134090 cites W2042411476 @default.
• W2514134090 cites W2044465436 @default.
• W2514134090 cites W2045474288 @default.
• W2514134090 cites W2049244267 @default.
• W2514134090 cites W2050178956 @default.
• W2514134090 cites W2050966312 @default.
• W2514134090 cites W2051560414 @default.
• W2514134090 cites W2052188391 @default.
• W2514134090 cites W2053598374 @default.
• W2514134090 cites W2054680310 @default.
• W2514134090 cites W2055823448 @default.
• W2514134090 cites W2056376829 @default.
• W2514134090 cites W2060003997 @default.
• W2514134090 cites W2060956030 @default.
• W2514134090 cites W2062406143 @default.
• W2514134090 cites W2066776235 @default.
• W2514134090 cites W2067541521 @default.
• W2514134090 cites W2070630942 @default.
• W2514134090 cites W2079948289 @default.
• W2514134090 cites W2081113495 @default.
• W2514134090 cites W2085083234 @default.
• W2514134090 cites W2087272783 @default.
• W2514134090 cites W2089710487 @default.
• W2514134090 cites W2095497087 @default.
• W2514134090 cites W2099175138 @default.
• W2514134090 cites W2102978920 @default.
• W2514134090 cites W2103789511 @default.
• W2514134090 cites W2106452452 @default.
• W2514134090 cites W2107768042 @default.
• W2514134090 cites W2109429564 @default.
• W2514134090 cites W2113398622 @default.
• W2514134090 cites W2113504642 @default.
• W2514134090 cites W2120303313 @default.
• W2514134090 cites W2123715983 @default.
• W2514134090 cites W2125209926 @default.
• W2514134090 cites W2134969640 @default.
• W2514134090 cites W2146109620 @default.
• W2514134090 cites W2147931773 @default.
• W2514134090 cites W2148609678 @default.
• W2514134090 cites W2152311269 @default.
• W2514134090 cites W2153231604 @default.
• W2514134090 cites W2163483547 @default.
• W2514134090 cites W2165006892 @default.
• W2514134090 cites W2168248023 @default.
• W2514134090 cites W2168337851 @default.
• W2514134090 cites W2183276865 @default.
• W2514134090 cites W2239915405 @default.
• W2514134090 cites W2314554900 @default.
• W2514134090 cites W2323136928 @default.
• W2514134090 cites W2342423373 @default.
• W2514134090 cites W2409570996 @default.
• W2514134090 doi "https://doi.org/10.1021/acs.biochem.6b00347" @default.
• W2514134090 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5356376" @default.

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