FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2514348568> ?p ?o ?g. }

• W2514348568 endingPage "21615" @default.
• W2514348568 startingPage "21607" @default.
• W2514348568 abstract "The high prevalence of house dust mite (HDM) allergy is a growing health problem worldwide, and the characterization of clinically important HDM allergens is a prerequisite for the development of diagnostic and therapeutic strategies. Here, we report a novel HDM allergen that belongs structurally to the highly conserved Rid/YjgF/YER057c/UK114 family (Rid family) with imine deaminase activity. Isolated HDM cDNA, named der f 34, encodes 128 amino acids homologous to Rid-like proteins. This new protein belongs to the Rid family and has seven conserved residues involved in enamine/imine deaminase activity. Indeed, we demonstrated that purified Der f 34 had imine deaminase activity that preferentially acted on leucine and methionine. Native Der f 34 showed a high IgE binding frequency as revealed by two-dimensional immunoblotting (62.5%) or ELISA (68%), which was comparable with those of a major HDM allergen Der f 2 (77.5 and 79%, respectively). We also found that Der f 34 showed cross-reactivity with another prominent indoor allergen source, Aspergillus fumigatus. This is the first report showing that the Rid family imine deaminase represents an additional important pan-allergen that is conserved across organisms. The high prevalence of house dust mite (HDM) allergy is a growing health problem worldwide, and the characterization of clinically important HDM allergens is a prerequisite for the development of diagnostic and therapeutic strategies. Here, we report a novel HDM allergen that belongs structurally to the highly conserved Rid/YjgF/YER057c/UK114 family (Rid family) with imine deaminase activity. Isolated HDM cDNA, named der f 34, encodes 128 amino acids homologous to Rid-like proteins. This new protein belongs to the Rid family and has seven conserved residues involved in enamine/imine deaminase activity. Indeed, we demonstrated that purified Der f 34 had imine deaminase activity that preferentially acted on leucine and methionine. Native Der f 34 showed a high IgE binding frequency as revealed by two-dimensional immunoblotting (62.5%) or ELISA (68%), which was comparable with those of a major HDM allergen Der f 2 (77.5 and 79%, respectively). We also found that Der f 34 showed cross-reactivity with another prominent indoor allergen source, Aspergillus fumigatus. This is the first report showing that the Rid family imine deaminase represents an additional important pan-allergen that is conserved across organisms." @default.
• W2514348568 created "2016-09-16" @default.
• W2514348568 creator A5012272657 @default.
• W2514348568 creator A5026612909 @default.
• W2514348568 creator A5029423637 @default.
• W2514348568 creator A5030979719 @default.
• W2514348568 creator A5036274299 @default.
• W2514348568 creator A5037295228 @default.
• W2514348568 creator A5042656740 @default.
• W2514348568 creator A5052187169 @default.
• W2514348568 creator A5060342288 @default.
• W2514348568 creator A5061476367 @default.
• W2514348568 creator A5072844494 @default.
• W2514348568 creator A5074203320 @default.
• W2514348568 creator A5074701942 @default.
• W2514348568 creator A5077619843 @default.
• W2514348568 creator A5079328051 @default.
• W2514348568 creator A5080419281 @default.
• W2514348568 creator A5083418135 @default.
• W2514348568 creator A5091264293 @default.
• W2514348568 date "2016-10-01" @default.
• W2514348568 modified "2023-10-18" @default.
• W2514348568 title "Der f 34, a Novel Major House Dust Mite Allergen Belonging to a Highly Conserved Rid/YjgF/YER057c/UK114 Family of Imine Deaminases" @default.
• W2514348568 cites W1508046341 @default.
• W2514348568 cites W1548476693 @default.
• W2514348568 cites W1589829943 @default.
• W2514348568 cites W1844010541 @default.
• W2514348568 cites W1856030288 @default.
• W2514348568 cites W1873842403 @default.
• W2514348568 cites W1887341968 @default.
• W2514348568 cites W1893294895 @default.
• W2514348568 cites W1959647660 @default.
• W2514348568 cites W1966000410 @default.
• W2514348568 cites W1974988105 @default.
• W2514348568 cites W1976751265 @default.
• W2514348568 cites W1977008844 @default.
• W2514348568 cites W1982443628 @default.
• W2514348568 cites W1987434147 @default.
• W2514348568 cites W2010102645 @default.
• W2514348568 cites W2030827577 @default.
• W2514348568 cites W2032436934 @default.
• W2514348568 cites W2035981733 @default.
• W2514348568 cites W2038286480 @default.
• W2514348568 cites W2041311316 @default.
• W2514348568 cites W2041891125 @default.
• W2514348568 cites W2049687832 @default.
• W2514348568 cites W2052280634 @default.
• W2514348568 cites W2055848875 @default.
• W2514348568 cites W2057514257 @default.
• W2514348568 cites W2059359155 @default.
• W2514348568 cites W2068828230 @default.
• W2514348568 cites W2072284792 @default.
• W2514348568 cites W2077080163 @default.
• W2514348568 cites W2084408826 @default.
• W2514348568 cites W2085540738 @default.
• W2514348568 cites W2087390644 @default.
• W2514348568 cites W2103313979 @default.
• W2514348568 cites W2108281766 @default.
• W2514348568 cites W2114757629 @default.
• W2514348568 cites W2151544982 @default.
• W2514348568 cites W2153034426 @default.
• W2514348568 cites W2157728190 @default.
• W2514348568 cites W2163283525 @default.
• W2514348568 cites W2166974823 @default.
• W2514348568 cites W2169173043 @default.
• W2514348568 cites W2171580076 @default.
• W2514348568 cites W2272379762 @default.
• W2514348568 cites W2404127895 @default.
• W2514348568 cites W4366064412 @default.
• W2514348568 cites W2159824191 @default.
• W2514348568 doi "https://doi.org/10.1074/jbc.m116.728006" @default.
• W2514348568 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5076831" @default.
• W2514348568 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27539850" @default.
• W2514348568 hasPublicationYear "2016" @default.
• W2514348568 type Work @default.
• W2514348568 sameAs 2514348568 @default.
• W2514348568 citedByCount "17" @default.
• W2514348568 countsByYear W25143485682017 @default.
• W2514348568 countsByYear W25143485682018 @default.
• W2514348568 countsByYear W25143485682019 @default.
• W2514348568 countsByYear W25143485682020 @default.
• W2514348568 countsByYear W25143485682021 @default.
• W2514348568 countsByYear W25143485682022 @default.
• W2514348568 countsByYear W25143485682023 @default.
• W2514348568 crossrefType "journal-article" @default.
• W2514348568 hasAuthorship W2514348568A5012272657 @default.
• W2514348568 hasAuthorship W2514348568A5026612909 @default.
• W2514348568 hasAuthorship W2514348568A5029423637 @default.
• W2514348568 hasAuthorship W2514348568A5030979719 @default.
• W2514348568 hasAuthorship W2514348568A5036274299 @default.
• W2514348568 hasAuthorship W2514348568A5037295228 @default.
• W2514348568 hasAuthorship W2514348568A5042656740 @default.
• W2514348568 hasAuthorship W2514348568A5052187169 @default.
• W2514348568 hasAuthorship W2514348568A5060342288 @default.
• W2514348568 hasAuthorship W2514348568A5061476367 @default.
• W2514348568 hasAuthorship W2514348568A5072844494 @default.
• W2514348568 hasAuthorship W2514348568A5074203320 @default.
• W2514348568 hasAuthorship W2514348568A5074701942 @default.

• next