FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2514501984> ?p ?o ?g. }

• W2514501984 abstract "Embryonic stem cells (ESC) are derived from the inner cell mass (ICM) of the blastocyst and have the capacity for unlimited proliferation while retaining their potential to differentiate into a wide variety of cell types when cultured in vitro. These properties have made of human embryonic stem cells (hESC) an excellent model on which to study the conditions required for differentiation into specific cell lineages, and consequently the possibility of transplanting specific cell types into damaged tissues. The continued turn over of ESC while maintaining an undifferentiated state is dependent on unusual cell cycle properties. These unusual proliferative properties are responsible for the generation of tumours when these cells are injected into adult animals. Thus, the study of the unusual proliferative properties of hESC needs to be addressed if their potential is to be realized. To date, most studies of the cell cycle in hESC have been descriptive, lacking functional studies that reveal the mechanisms of how the cell cycle maintains pluripotency and self- renewal of hESC. In this thesis we sought to understand the mechanisms of cell cycle control of hESC. We asked the question if a single cell cycle gene could regulate the self-renewal or pluripotency properties of hESC using a gain and loss of gene function strategy. We have identified that the protein expression of the p27Kip1 cell cycle inhibitor was low in human pluripotent cells, but its expression increased during differentiation together with changes in the cell cycle structure of pluripotent cells. By adopting a gain and loss of function strategy we increased or reduced its expression in undifferentiating conditions to define its functional role in self-renewal and pluripotency of Hesc, using undifferentiation conditions, overexpression of p27Kip1 in hESC lead to a G1 phase arrest with an enlarged and flattened hESC morphology and consequently loss of self-renewal ability. Loss of p27Kip1 caused an increase of self-renewal while maintaining an undifferentiated phenotype. Moreover, we have shown that a change in the balance of p27Kip1 levels in undifferentiated hESC affects expression of the mesoderm markers: BRACHYURY and TWIST. We have found that expression changes of TWIST are associated with the presence of p27Kip1 protein in the TWIST1 gene promoter. The results presented in this thesis have interesting implications in stem cell biology. Firstly, these results define that the maintenance of p27Kip1 protein levels at a certain level is essential for self-renewal and pluripotency of hESC. Secondly, p27Kip1 is involved in the regulation of TWIST which is upregulated in several types of tumours and induces an epithelial-mesenchymal transition to facilitate tumor metastasis." @default.
• W2514501984 created "2016-09-16" @default.
• W2514501984 creator A5015938754 @default.
• W2514501984 date "2011-06-09" @default.
• W2514501984 modified "2023-09-27" @default.
• W2514501984 title "The Role of the Cell Cycle in Human Embryonic Stem Cell Self-Renewal and Pluripotency (La función del ciclo celular en la auto-renovación y la pluripotencia de las células madre embrionarias humanas)" @default.
• W2514501984 hasPublicationYear "2011" @default.
• W2514501984 type Work @default.
• W2514501984 sameAs 2514501984 @default.
• W2514501984 citedByCount "0" @default.
• W2514501984 crossrefType "journal-article" @default.
• W2514501984 hasAuthorship W2514501984A5015938754 @default.
• W2514501984 hasConcept C104317684 @default.
• W2514501984 hasConcept C107459253 @default.
• W2514501984 hasConcept C143948831 @default.
• W2514501984 hasConcept C145103041 @default.
• W2514501984 hasConcept C148738053 @default.
• W2514501984 hasConcept C1491633281 @default.
• W2514501984 hasConcept C27390768 @default.
• W2514501984 hasConcept C28328180 @default.
• W2514501984 hasConcept C29537977 @default.
• W2514501984 hasConcept C54355233 @default.
• W2514501984 hasConcept C86803240 @default.
• W2514501984 hasConcept C95444343 @default.
• W2514501984 hasConceptScore W2514501984C104317684 @default.
• W2514501984 hasConceptScore W2514501984C107459253 @default.
• W2514501984 hasConceptScore W2514501984C143948831 @default.
• W2514501984 hasConceptScore W2514501984C145103041 @default.
• W2514501984 hasConceptScore W2514501984C148738053 @default.
• W2514501984 hasConceptScore W2514501984C1491633281 @default.
• W2514501984 hasConceptScore W2514501984C27390768 @default.
• W2514501984 hasConceptScore W2514501984C28328180 @default.
• W2514501984 hasConceptScore W2514501984C29537977 @default.
• W2514501984 hasConceptScore W2514501984C54355233 @default.
• W2514501984 hasConceptScore W2514501984C86803240 @default.
• W2514501984 hasConceptScore W2514501984C95444343 @default.
• W2514501984 hasLocation W25145019841 @default.
• W2514501984 hasOpenAccess W2514501984 @default.
• W2514501984 hasPrimaryLocation W25145019841 @default.
• W2514501984 hasRelatedWork W1588898520 @default.
• W2514501984 hasRelatedWork W169482959 @default.
• W2514501984 hasRelatedWork W1975799446 @default.
• W2514501984 hasRelatedWork W2008635881 @default.
• W2514501984 hasRelatedWork W2009407800 @default.
• W2514501984 hasRelatedWork W2034276834 @default.
• W2514501984 hasRelatedWork W2042850071 @default.
• W2514501984 hasRelatedWork W2048535316 @default.
• W2514501984 hasRelatedWork W2053457288 @default.
• W2514501984 hasRelatedWork W2059098560 @default.
• W2514501984 hasRelatedWork W2066632404 @default.
• W2514501984 hasRelatedWork W2138523762 @default.
• W2514501984 hasRelatedWork W2138908905 @default.
• W2514501984 hasRelatedWork W2140780283 @default.
• W2514501984 hasRelatedWork W2147476207 @default.
• W2514501984 hasRelatedWork W2247954957 @default.
• W2514501984 hasRelatedWork W2418807767 @default.
• W2514501984 hasRelatedWork W2798042600 @default.
• W2514501984 hasRelatedWork W3002712193 @default.
• W2514501984 hasRelatedWork W3082189815 @default.
• W2514501984 isParatext "false" @default.
• W2514501984 isRetracted "false" @default.
• W2514501984 magId "2514501984" @default.
• W2514501984 workType "article" @default.