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- W2514513014 abstract "Glypican-5 (GPC5) is an important tumor suppressor, while little is known about the impact of GPC5 on proliferation ability and gene expression in lung adenocarcinoma cell lines. Here, we stably overexpressed GPC5 in A549 cells and investigated the impact of cell proliferation ability and gene expression.A549 cells that stably overexpressed GPC5 were constructed by lentivirus. Cell counter kit 8 (CCK8), colony formation, EdU assay were conducted to analyze cell proliferation ability, and transcriptome sequencing was utilized to investigate gene expression profile.CCK8 assay showed that compared with empty vector, overexpression of GPC5 significantly inhibited cell proliferation rate in A549 cells and the number of colony was also decreased (181±17 vs 278±23). EdU assay also confirmed the percentage of positive staining cells decreased after GPC5 overexpression. Transcriptome sequencing revealed that 2,108 genes were differentially expressed after GPC5 overexpression. Among these differentially expressed genes, 47 genes of the Gene Ontology item positive regulation of cell proliferation were downregulated.Overexpression of GPC5 inhibited proliferation ability in lung adenocarcinoma A549 cells and genes with the function of positive regulation of cell proliferation were downregulated.背景与目的 磷脂酰肌醇蛋白聚糖-5（glypican-5, GPC5）是一个重要的抑癌基因，然而GPC5对肺腺癌细胞增殖能力和基因表达的影响目前研究甚少。本研究拟在肺腺癌A549细胞中过表达GPC5以研究细胞增殖能力和基因表达变化情况。方法 通过慢病毒载体构建稳定过表达GPC5的A549细胞株，通过Cell Counter Kit 8 (CCK8)、平板克隆和EdU实验检测细胞增殖能力；通过高通量转录组测序研究基因表达变化。结果 相对于空白载体组，CCK8实验发现过表达GPC5可以明显抑制A549细胞的增殖速率；平板克隆实验结果显示，过表达GPC5之后A549细胞克隆形成能力下降（181±17 vs 278±23）；EdU染色结果显示过表达GPC5后阳性染色细胞比例下降。转录组测序结果提示过表达GPC5之后，2,108个基因表达发生明显变化，其中具有正性调节细胞增殖作用的基因明显下调。结论 过表达GPC5可以明显抑制肺腺癌细A549的增殖能力，而且过表达GPC5后具有正性调节细胞增殖作用的基因表达下调。." @default.
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- W2514513014 date "2016-08-20" @default.
- W2514513014 modified "2023-09-25" @default.
- W2514513014 title "[Investigation of Gene Expression Profile of A549 Cells after Overexpression of GPC5 by High Throughput Transcriptome Sequencing]." @default.
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- W2514513014 doi "https://doi.org/10.3779/j.issn.1009-3419.2016.08.11" @default.
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