FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2514542591> ?p ?o ?g. }

• W2514542591 endingPage "C1899" @default.
• W2514542591 startingPage "C1889" @default.
• W2514542591 abstract "Using the Xenopus oocyte expression system, we examined the mechanisms by which the beta- and gamma-subunits of an epithelial Na+ channel (ENaC) regulate alpha-subunit channel activity and the mechanisms by which beta-subunit truncations cause ENaC activation. Expression of alpha-ENaC alone produced small amiloride-sensitive currents (-43 +/- 10 nA, n = 7). These currents increased > 30-fold with the coexpression of beta- and gamma-ENaC to -1,476 +/- 254 nA (n = 20). This increase was accompanied by a 3.1- and 2.7-fold increase of membrane fluorescence intensity in the animal and vegetal poles of the oocyte, respectively, with use of an antibody directed against the alpha-subunit of ENaC. Truncation of the last 75 amino acids of the beta-subunit COOH terminus, as found in the original pedigree of individuals with Liddle's syndrome, caused a 4.4-fold (n = 17) increase of the amiloride-sensitive currents compared with wild-type alpha beta gamma-ENaC. This was accompanied by a 35% increase of animal pole membrane fluorescence intensity. Injection of a 30-amino acid peptide with sequence identity to the COOH terminus of the human beta-ENaC significantly reduced the amiloride-sensitive currents by 40-50%. These observations suggest a tonic inhibitory role on the channel's open probability (Po) by the COOH terminus of beta-ENaC. We conclude that the changes of current observed with coexpression of the beta- and gamma-subunits or those observed with beta-subunit truncation are likely the result of changes of channel density in combination with large changes of Po." @default.
• W2514542591 created "2016-09-16" @default.
• W2514542591 creator A5008621245 @default.
• W2514542591 creator A5055851900 @default.
• W2514542591 creator A5060448700 @default.
• W2514542591 date "1997-12-01" @default.
• W2514542591 modified "2023-09-23" @default.
• W2514542591 title "Regulation of a cloned epithelial Na⁺channel by its β- and γ-subunits" @default.
• W2514542591 cites W1506512444 @default.
• W2514542591 cites W1541018744 @default.
• W2514542591 cites W174043930 @default.
• W2514542591 cites W1850052335 @default.
• W2514542591 cites W1887182106 @default.
• W2514542591 cites W1954315298 @default.
• W2514542591 cites W1966571419 @default.
• W2514542591 cites W1968226795 @default.
• W2514542591 cites W1977987849 @default.
• W2514542591 cites W2005579207 @default.
• W2514542591 cites W2009063887 @default.
• W2514542591 cites W2024661464 @default.
• W2514542591 cites W2043421138 @default.
• W2514542591 cites W2043502742 @default.
• W2514542591 cites W2064864270 @default.
• W2514542591 cites W2073881942 @default.
• W2514542591 cites W2074331481 @default.
• W2514542591 cites W2078119356 @default.
• W2514542591 cites W2079573668 @default.
• W2514542591 cites W2087499467 @default.
• W2514542591 cites W2090117730 @default.
• W2514542591 cites W2133136550 @default.
• W2514542591 cites W2140853270 @default.
• W2514542591 cites W2224842634 @default.
• W2514542591 doi "https://doi.org/10.1152/ajpcell.1997.273.6.c1889" @default.
• W2514542591 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9435494" @default.
• W2514542591 hasPublicationYear "1997" @default.
• W2514542591 type Work @default.
• W2514542591 sameAs 2514542591 @default.
• W2514542591 citedByCount "42" @default.
• W2514542591 countsByYear W25145425912012 @default.
• W2514542591 countsByYear W25145425912013 @default.
• W2514542591 countsByYear W25145425912016 @default.
• W2514542591 countsByYear W25145425912022 @default.
• W2514542591 crossrefType "journal-article" @default.
• W2514542591 hasAuthorship W2514542591A5008621245 @default.
• W2514542591 hasAuthorship W2514542591A5055851900 @default.
• W2514542591 hasAuthorship W2514542591A5060448700 @default.
• W2514542591 hasConcept C104292427 @default.
• W2514542591 hasConcept C104317684 @default.
• W2514542591 hasConcept C12554922 @default.
• W2514542591 hasConcept C126322002 @default.
• W2514542591 hasConcept C134018914 @default.
• W2514542591 hasConcept C153911025 @default.
• W2514542591 hasConcept C178790620 @default.
• W2514542591 hasConcept C185592680 @default.
• W2514542591 hasConcept C199360897 @default.
• W2514542591 hasConcept C2776174256 @default.
• W2514542591 hasConcept C2779535977 @default.
• W2514542591 hasConcept C2779608910 @default.
• W2514542591 hasConcept C41008148 @default.
• W2514542591 hasConcept C515207424 @default.
• W2514542591 hasConcept C537181965 @default.
• W2514542591 hasConcept C55493867 @default.
• W2514542591 hasConcept C6507245 @default.
• W2514542591 hasConcept C71924100 @default.
• W2514542591 hasConcept C83730767 @default.
• W2514542591 hasConcept C86803240 @default.
• W2514542591 hasConceptScore W2514542591C104292427 @default.
• W2514542591 hasConceptScore W2514542591C104317684 @default.
• W2514542591 hasConceptScore W2514542591C12554922 @default.
• W2514542591 hasConceptScore W2514542591C126322002 @default.
• W2514542591 hasConceptScore W2514542591C134018914 @default.
• W2514542591 hasConceptScore W2514542591C153911025 @default.
• W2514542591 hasConceptScore W2514542591C178790620 @default.
• W2514542591 hasConceptScore W2514542591C185592680 @default.
• W2514542591 hasConceptScore W2514542591C199360897 @default.
• W2514542591 hasConceptScore W2514542591C2776174256 @default.
• W2514542591 hasConceptScore W2514542591C2779535977 @default.
• W2514542591 hasConceptScore W2514542591C2779608910 @default.
• W2514542591 hasConceptScore W2514542591C41008148 @default.
• W2514542591 hasConceptScore W2514542591C515207424 @default.
• W2514542591 hasConceptScore W2514542591C537181965 @default.
• W2514542591 hasConceptScore W2514542591C55493867 @default.
• W2514542591 hasConceptScore W2514542591C6507245 @default.
• W2514542591 hasConceptScore W2514542591C71924100 @default.
• W2514542591 hasConceptScore W2514542591C83730767 @default.
• W2514542591 hasConceptScore W2514542591C86803240 @default.
• W2514542591 hasIssue "6" @default.
• W2514542591 hasLocation W25145425911 @default.
• W2514542591 hasLocation W25145425912 @default.
• W2514542591 hasOpenAccess W2514542591 @default.
• W2514542591 hasPrimaryLocation W25145425911 @default.
• W2514542591 hasRelatedWork W1787932272 @default.
• W2514542591 hasRelatedWork W1980540394 @default.
• W2514542591 hasRelatedWork W2026914265 @default.
• W2514542591 hasRelatedWork W2038245560 @default.
• W2514542591 hasRelatedWork W2090617123 @default.
• W2514542591 hasRelatedWork W2184172377 @default.
• W2514542591 hasRelatedWork W2410792400 @default.

• next