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- W2514732651 abstract "The aim of this study is to investigate the effects of parenteral glutamine(GLN) supplementation combined with enteral nutrition (EN) on heat shock protein 90(Hsp90) expression, apoptosis of lymphoid organs and circulating lymphocytes, immunological function and survival in severely burned rats.Male SD rats were randomly assigned into 4 groups: a sham burn+EN+GLN-free amino acid (AA) group (n=10), a sham burn+EN+GLN group (n=10), a burn+EN+AA group (n=10), and a burn +EN +GLN group (n=10). Two hours after a 30% total body surface area (TBSA), full-thickness scald burn injury on the back was made, the burned rats in two experimental groups (the burn+EN+AA group and the burn+EN +GLN group) were fed with a conventional enteral nutrition solution by oral gavage for 7 days. Simultaneously, the rats in the burn+EN+GLN group were given 0.35g GLN/kg body weight/day once via a tail vein injection for 7 days, whereas those in the burn+EN+AA group were administered isocaloric/isonitrogenous GLN-free amino acid solution (Tyrosine) for comparison. The rats in two sham burn control groups (the sham burn+EN+AA group and the sham burn+EN +GLN group) were treated in the same procedure as above, except for burn injury. All rats in each of the four groups were given 175kcal/kg body wt/day. There was isonitrogenous, isovolumic and isocaloric intake among four groups. At the end of the 7th day after nutritional programme were finished, all rats were anesthetized and samples were collected for further analysis. Serum immunoglobulin quantification was conducted by ELISA. Circulating lymphocyte numbers were counted by Coulter LH-750 Analyzer. The percentages and apoptotic ratio of CD4 and CD8T lymphocytes in circulation were determined by flow cytometry (FCM). The neutrophil phagocytosis index (NPI) was examined. The GLN concentrations in plasma, thymus, spleen and skeletal muscle were measured by high performance liquid chromatography (HPLC). The organ index evaluation and TUNEL analysis of thymus and spleen were carried out. The expression of Hsp90 in thymus and spleen was analyzed by western blotting. Moreover, the survival in burned rats was observed.The results revealed that parenteral GLN supplementation combined with EN significantly increased the GLN concentrations of plasma and tissues, the serum immunoglobulin content, the circulating lymphocyte number, the CD4/CD8 ratio, the indexes of thymus and spleen, NPI and survival as compared with the burn+EN+AA group (p<0.05). The expression of Hsp90 in thymus and spleen in the burn+EN+GLN group was significantly up-regulated as compared with the burn+EN+AA group (p<0.05). The apoptosis in circulating CD4 and CD8 lymphocytes, thymus and spleen in the burn+EN+GLN group was significantly decreased as compared with the burn+EN+AA group (p<0.05).The results of this study show that parenteral GLN supplementation combined with EN may increase the GLN concentrations of plasma and tissues, up-regulate the expression of Hsp90, attenuate apoptosis in lymphoid organ and circulating lymphocyte, enhance the immunological function and improve survival in severely burned rats. Clinically, therapeutic efforts at the modulation of the immune dysfunction may contribute to a favorable outcome in severely burned patients." @default.
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- W2514732651 date "2016-11-01" @default.
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- W2514732651 title "Effect of parenteral glutamine supplementation combined with enteral nutrition on Hsp90 expression and lymphoid organ apoptosis in severely burned rats" @default.
- W2514732651 cites W1488866398 @default.
- W2514732651 cites W1567631042 @default.
- W2514732651 cites W1926790978 @default.
- W2514732651 cites W1929858239 @default.
- W2514732651 cites W1969152259 @default.
- W2514732651 cites W1973646697 @default.
- W2514732651 cites W1975729296 @default.
- W2514732651 cites W1977728367 @default.
- W2514732651 cites W1977905350 @default.
- W2514732651 cites W1981022377 @default.
- W2514732651 cites W1981158355 @default.
- W2514732651 cites W1982046231 @default.
- W2514732651 cites W1989184701 @default.
- W2514732651 cites W1996582484 @default.
- W2514732651 cites W1996659057 @default.
- W2514732651 cites W1997070121 @default.
- W2514732651 cites W2005458740 @default.
- W2514732651 cites W2007827117 @default.
- W2514732651 cites W2011376400 @default.
- W2514732651 cites W2017718664 @default.
- W2514732651 cites W2019508505 @default.
- W2514732651 cites W2026393795 @default.
- W2514732651 cites W2026659710 @default.
- W2514732651 cites W2028883591 @default.
- W2514732651 cites W2030933187 @default.
- W2514732651 cites W2034325617 @default.
- W2514732651 cites W2040339656 @default.
- W2514732651 cites W2048238764 @default.
- W2514732651 cites W2052667619 @default.
- W2514732651 cites W2054803214 @default.
- W2514732651 cites W2061402985 @default.
- W2514732651 cites W2065565919 @default.
- W2514732651 cites W2070158632 @default.
- W2514732651 cites W2074519302 @default.
- W2514732651 cites W2075414443 @default.
- W2514732651 cites W2077300226 @default.
- W2514732651 cites W2080372464 @default.
- W2514732651 cites W2080468426 @default.
- W2514732651 cites W2082625194 @default.
- W2514732651 cites W2084012675 @default.
- W2514732651 cites W2088109563 @default.
- W2514732651 cites W2088666798 @default.
- W2514732651 cites W2089339808 @default.
- W2514732651 cites W2092781537 @default.
- W2514732651 cites W2094690950 @default.
- W2514732651 cites W2102612880 @default.
- W2514732651 cites W2107086964 @default.
- W2514732651 cites W2108976949 @default.
- W2514732651 cites W2109383171 @default.
- W2514732651 cites W2119903505 @default.
- W2514732651 cites W2120420335 @default.
- W2514732651 cites W2121796720 @default.
- W2514732651 cites W2122476274 @default.
- W2514732651 cites W2122890361 @default.
- W2514732651 cites W2137296011 @default.
- W2514732651 cites W2139703406 @default.
- W2514732651 cites W2147552401 @default.
- W2514732651 cites W2152527970 @default.
- W2514732651 cites W2157448606 @default.
- W2514732651 cites W2185277106 @default.
- W2514732651 cites W2319289477 @default.
- W2514732651 cites W2321860467 @default.
- W2514732651 cites W2334750907 @default.
- W2514732651 cites W4229714404 @default.
- W2514732651 doi "https://doi.org/10.1016/j.burns.2016.02.009" @default.
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