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• W2515073872 endingPage "035017" @default.
• W2515073872 startingPage "035017" @default.
• W2515073872 abstract "The objective of this study is to design a novel and efficient portable lab-on-a-CD (LOCD) microfluidic device for separation of specific cells (target cells) using magnetic beads. In this study the results are shown for neutrophils as target cells. However, other kinds of target cells can be separated in a similar approach. The designed microfluidics can be utilized as a point of care system for neutrophil detection. This microfluidic system employs centrifugal and magnetic forces for separation. After model validation by the experimental data in the literature (that may be used as a design tool for developing centrifugo-magnetophoretic devices), two models are presented for separation of target cells using magnetic beads. The first model consists of one container in the inlet section and two containers in the outlets. Initially, the inlet container is filled with diluted blood sample which is a mixture of red blood cells (RBCs) plus neutrophils which are attached to Magnetic beads. It is shown that by using centrifugal and magnetic forces, this model can separate all neutrophils with recovery factor of ~100%. In the second model, due to excess of magnetic beads in usual experimental analysis (to ensure that all target cells are attached to them) the geometry is improved by adding a third outlet for these free magnetic beads. It is shown that at angular velocity of 45 rad s−1, recovery factor of 100% is achievable for RBCs, free magnetic beads and neutrophils as target cells." @default.
• W2515073872 created "2016-09-16" @default.
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• W2515073872 date "2016-02-09" @default.
• W2515073872 modified "2023-10-18" @default.
• W2515073872 title "Designing and modeling a centrifugal microfluidic device to separate target blood cells" @default.
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• W2515073872 doi "https://doi.org/10.1088/0960-1317/26/3/035017" @default.
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