FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2515202955> ?p ?o ?g. }

• W2515202955 endingPage "3069" @default.
• W2515202955 startingPage "3060" @default.
• W2515202955 abstract "ABSTRACT As type II fatty acid synthesis is essential for the growth of Escherichia coli , its many components are regarded as potential targets for novel antibacterial drugs. Among them, β-ketoacyl-acyl carrier protein (ACP) synthase (KAS) FabB is the exclusive factor for elongation of the cis -3-decenoyl-ACP ( cis -3-C 10 -ACP). In our previous study, we presented evidence to suggest that this may not be the case in Shewanella oneidensis , an emerging model gammaproteobacterium renowned for its respiratory versatility. Here, we identified FabF1, another KAS, as a functional replacement for FabB in S. oneidensis . In fabB + or desA + (encoding a desaturase) cells, which are capable of making unsaturated fatty acids (UFA), FabF1 is barely produced. However, UFA auxotroph mutants devoid of both fabB and desA genes can be spontaneously converted to suppressor strains, which no longer require exogenous UFAs for growth. Suppression is caused by a TGTTTT deletion in the region upstream of the fabF1 gene, resulting in enhanced FabF1 production. We further demonstrated that the deletion leads to transcription read-through of the terminator for acpP , an acyl carrier protein gene immediately upstream of fabF1 . There are multiple tandem repeats in the region covering the terminator, and the TGTTTT deletion, as well as others, compromises the terminator efficacy. In addition, FabF2 also shows an ability to complement the FabB loss, albeit substantially less effectively than FabF1. IMPORTANCE It has been firmly established that FabB for UFA synthesis via type II fatty acid synthesis in FabA-containing bacteria such as E. coli is essential. However, S. oneidensis appears to be an exception. In this bacterium, FabF1, when sufficiently expressed, is able to fully complement the FabB loss. Importantly, such a capability can be obtained by spontaneous mutations, which lead to transcription read-through. Therefore, our data, by identifying the functional overlap between FabB and FabFs, provide new insights into the current understanding of KAS and help reveal novel ways to block UFA synthesis for therapeutic purposes." @default.
• W2515202955 created "2016-09-16" @default.
• W2515202955 creator A5010331798 @default.
• W2515202955 creator A5023568877 @default.
• W2515202955 creator A5024809872 @default.
• W2515202955 creator A5026204677 @default.
• W2515202955 creator A5041573027 @default.
• W2515202955 date "2016-11-15" @default.
• W2515202955 modified "2023-10-15" @default.
• W2515202955 title "Suppression of <i>fabB</i> Mutation by <i>fabF1</i> Is Mediated by Transcription Read-through in Shewanella oneidensis" @default.
• W2515202955 cites W1497680908 @default.
• W2515202955 cites W1510623452 @default.
• W2515202955 cites W1530145064 @default.
• W2515202955 cites W1548129143 @default.
• W2515202955 cites W1560135653 @default.
• W2515202955 cites W1577856817 @default.
• W2515202955 cites W1699689362 @default.
• W2515202955 cites W1945353891 @default.
• W2515202955 cites W1957500988 @default.
• W2515202955 cites W1993563214 @default.
• W2515202955 cites W1994328828 @default.
• W2515202955 cites W2003007485 @default.
• W2515202955 cites W2004432131 @default.
• W2515202955 cites W2005597552 @default.
• W2515202955 cites W2008386225 @default.
• W2515202955 cites W2016186068 @default.
• W2515202955 cites W2024610718 @default.
• W2515202955 cites W2026182346 @default.
• W2515202955 cites W2028694104 @default.
• W2515202955 cites W2029622458 @default.
• W2515202955 cites W2035318758 @default.
• W2515202955 cites W2062612117 @default.
• W2515202955 cites W2087404650 @default.
• W2515202955 cites W2093683102 @default.
• W2515202955 cites W2095885803 @default.
• W2515202955 cites W2099450313 @default.
• W2515202955 cites W2102004106 @default.
• W2515202955 cites W2107170880 @default.
• W2515202955 cites W2108465503 @default.
• W2515202955 cites W2116137883 @default.
• W2515202955 cites W2125601425 @default.
• W2515202955 cites W2138762386 @default.
• W2515202955 cites W2139295216 @default.
• W2515202955 cites W2140927174 @default.
• W2515202955 cites W2144047802 @default.
• W2515202955 cites W2149459456 @default.
• W2515202955 cites W2152227548 @default.
• W2515202955 cites W2152404025 @default.
• W2515202955 cites W2158483402 @default.
• W2515202955 cites W21624655 @default.
• W2515202955 cites W2163451286 @default.
• W2515202955 cites W2165889806 @default.
• W2515202955 cites W2328799290 @default.
• W2515202955 cites W2338102386 @default.
• W2515202955 cites W2419133522 @default.
• W2515202955 doi "https://doi.org/10.1128/jb.00463-16" @default.
• W2515202955 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5075035" @default.
• W2515202955 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27573012" @default.
• W2515202955 hasPublicationYear "2016" @default.
• W2515202955 type Work @default.
• W2515202955 sameAs 2515202955 @default.
• W2515202955 citedByCount "13" @default.
• W2515202955 countsByYear W25152029552017 @default.
• W2515202955 countsByYear W25152029552018 @default.
• W2515202955 countsByYear W25152029552019 @default.
• W2515202955 countsByYear W25152029552020 @default.
• W2515202955 countsByYear W25152029552021 @default.
• W2515202955 countsByYear W25152029552022 @default.
• W2515202955 crossrefType "journal-article" @default.
• W2515202955 hasAuthorship W2515202955A5010331798 @default.
• W2515202955 hasAuthorship W2515202955A5023568877 @default.
• W2515202955 hasAuthorship W2515202955A5024809872 @default.
• W2515202955 hasAuthorship W2515202955A5026204677 @default.
• W2515202955 hasAuthorship W2515202955A5041573027 @default.
• W2515202955 hasBestOaLocation W25152029551 @default.
• W2515202955 hasConcept C104317684 @default.
• W2515202955 hasConcept C116403925 @default.
• W2515202955 hasConcept C121332964 @default.
• W2515202955 hasConcept C1276947 @default.
• W2515202955 hasConcept C143065580 @default.
• W2515202955 hasConcept C178046730 @default.
• W2515202955 hasConcept C20950039 @default.
• W2515202955 hasConcept C2780786045 @default.
• W2515202955 hasConcept C2781170380 @default.
• W2515202955 hasConcept C523546767 @default.
• W2515202955 hasConcept C54355233 @default.
• W2515202955 hasConcept C547475151 @default.
• W2515202955 hasConcept C55493867 @default.
• W2515202955 hasConcept C86803240 @default.
• W2515202955 hasConcept C89423630 @default.
• W2515202955 hasConceptScore W2515202955C104317684 @default.
• W2515202955 hasConceptScore W2515202955C116403925 @default.
• W2515202955 hasConceptScore W2515202955C121332964 @default.
• W2515202955 hasConceptScore W2515202955C1276947 @default.
• W2515202955 hasConceptScore W2515202955C143065580 @default.
• W2515202955 hasConceptScore W2515202955C178046730 @default.
• W2515202955 hasConceptScore W2515202955C20950039 @default.
• W2515202955 hasConceptScore W2515202955C2780786045 @default.

• next