FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2515224443> ?p ?o ?g. }

• W2515224443 abstract "Secreted proteins are reported to induce cell-mediated immunity characterised by the production of interferon-gamma (IFN)-γ. In this study three open reading frames (ORFs) (Erum8060, Erum7760, Erum5000) encoding secreted proteins were selected from the Ehrlichia ruminantium (Welgevonden) genome sequence using bioinformatics tools to determine whether they induce a cellular immune response in vitro with mononuclear cells from needle and tick infected animals. The whole recombinant protein of the three ORFs as well as four adjacent fragments of the Erum5000 protein (Erum5000A, Erum5000B, Erum5000C, Erum5000D) were successfully expressed in a bacterial expression system which was confirmed by immunoblots using anti-His antibodies and sheep sera. These recombinant proteins were assayed with immune sheep and cattle peripheral blood mononuclear cells (PBMCs), spleen and lymph node (LN) cells to determine whether they induce recall cellular immune responses in vitro. Significant proliferative responses and IFN-γ production were evident for all recombinant proteins, especially Erum5000A, in both ruminant species tested. Thus overlapping peptides spanning Erum5000A were synthesised and peptides that induce proliferation of memory CD4+ and CD8+ T cells and production of IFN-γ were identified. These results illustrate that a Th1 type immune response was elicited and these recombinant proteins and peptides may therefore be promising candidates for development of a heartwater vaccine." @default.
• W2515224443 created "2016-09-16" @default.
• W2515224443 creator A5029866693 @default.
• W2515224443 creator A5070574395 @default.
• W2515224443 creator A5078417026 @default.
• W2515224443 creator A5080107216 @default.
• W2515224443 creator A5081380686 @default.
• W2515224443 creator A5083668296 @default.
• W2515224443 date "2016-03-09" @default.
• W2515224443 modified "2023-10-16" @default.
• W2515224443 title "Cellular immune responses induced <i>in vitro</i> by <i>Ehrlichia ruminantium</i> secreted proteins and identification of vaccine candidate peptides" @default.
• W2515224443 cites W1213337631 @default.
• W2515224443 cites W1468118048 @default.
• W2515224443 cites W1502955003 @default.
• W2515224443 cites W1547018200 @default.
• W2515224443 cites W166590630 @default.
• W2515224443 cites W1969606774 @default.
• W2515224443 cites W1998567760 @default.
• W2515224443 cites W1999152416 @default.
• W2515224443 cites W2002237984 @default.
• W2515224443 cites W2038088213 @default.
• W2515224443 cites W2044376436 @default.
• W2515224443 cites W2060142297 @default.
• W2515224443 cites W2061583865 @default.
• W2515224443 cites W2065519739 @default.
• W2515224443 cites W2065588157 @default.
• W2515224443 cites W2071805896 @default.
• W2515224443 cites W2073256556 @default.
• W2515224443 cites W2076932010 @default.
• W2515224443 cites W2078011555 @default.
• W2515224443 cites W2098073190 @default.
• W2515224443 cites W2100138201 @default.
• W2515224443 cites W2106528225 @default.
• W2515224443 cites W2107472966 @default.
• W2515224443 cites W2118903939 @default.
• W2515224443 cites W2126085416 @default.
• W2515224443 cites W2131184698 @default.
• W2515224443 cites W2138488727 @default.
• W2515224443 cites W2143744986 @default.
• W2515224443 cites W2149217412 @default.
• W2515224443 cites W2169825678 @default.
• W2515224443 cites W4243577559 @default.
• W2515224443 doi "https://doi.org/10.4102/ojvr.v83i1.1170" @default.
• W2515224443 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6238801" @default.
• W2515224443 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27608502" @default.
• W2515224443 hasPublicationYear "2016" @default.
• W2515224443 type Work @default.
• W2515224443 sameAs 2515224443 @default.
• W2515224443 citedByCount "7" @default.
• W2515224443 countsByYear W25152244432017 @default.
• W2515224443 countsByYear W25152244432018 @default.
• W2515224443 countsByYear W25152244432019 @default.
• W2515224443 countsByYear W25152244432021 @default.
• W2515224443 countsByYear W25152244432023 @default.
• W2515224443 crossrefType "journal-article" @default.
• W2515224443 hasAuthorship W2515224443A5029866693 @default.
• W2515224443 hasAuthorship W2515224443A5070574395 @default.
• W2515224443 hasAuthorship W2515224443A5078417026 @default.
• W2515224443 hasAuthorship W2515224443A5080107216 @default.
• W2515224443 hasAuthorship W2515224443A5081380686 @default.
• W2515224443 hasAuthorship W2515224443A5083668296 @default.
• W2515224443 hasBestOaLocation W25152244431 @default.
• W2515224443 hasConcept C104317684 @default.
• W2515224443 hasConcept C137061746 @default.
• W2515224443 hasConcept C147483822 @default.
• W2515224443 hasConcept C159047783 @default.
• W2515224443 hasConcept C159654299 @default.
• W2515224443 hasConcept C167625842 @default.
• W2515224443 hasConcept C167672396 @default.
• W2515224443 hasConcept C202751555 @default.
• W2515224443 hasConcept C203014093 @default.
• W2515224443 hasConcept C2779053233 @default.
• W2515224443 hasConcept C2779620486 @default.
• W2515224443 hasConcept C2779657035 @default.
• W2515224443 hasConcept C2780530800 @default.
• W2515224443 hasConcept C40767141 @default.
• W2515224443 hasConcept C47289529 @default.
• W2515224443 hasConcept C54355233 @default.
• W2515224443 hasConcept C86803240 @default.
• W2515224443 hasConcept C8891405 @default.
• W2515224443 hasConcept C89423630 @default.
• W2515224443 hasConceptScore W2515224443C104317684 @default.
• W2515224443 hasConceptScore W2515224443C137061746 @default.
• W2515224443 hasConceptScore W2515224443C147483822 @default.
• W2515224443 hasConceptScore W2515224443C159047783 @default.
• W2515224443 hasConceptScore W2515224443C159654299 @default.
• W2515224443 hasConceptScore W2515224443C167625842 @default.
• W2515224443 hasConceptScore W2515224443C167672396 @default.
• W2515224443 hasConceptScore W2515224443C202751555 @default.
• W2515224443 hasConceptScore W2515224443C203014093 @default.
• W2515224443 hasConceptScore W2515224443C2779053233 @default.
• W2515224443 hasConceptScore W2515224443C2779620486 @default.
• W2515224443 hasConceptScore W2515224443C2779657035 @default.
• W2515224443 hasConceptScore W2515224443C2780530800 @default.
• W2515224443 hasConceptScore W2515224443C40767141 @default.
• W2515224443 hasConceptScore W2515224443C47289529 @default.
• W2515224443 hasConceptScore W2515224443C54355233 @default.
• W2515224443 hasConceptScore W2515224443C86803240 @default.
• W2515224443 hasConceptScore W2515224443C8891405 @default.
• W2515224443 hasConceptScore W2515224443C89423630 @default.

• next