Matches in SemOpenAlex for { <https://semopenalex.org/work/W2515447035> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W2515447035 abstract "Two forms of a-galactosidase (a-t-galactoside galactohydrolase, E.C. 3.2.1.22) which differed in molecular weight were resolved from Cucumis sativus L. leaves. The enzymes were partially purified using ammonium sulfate fractionation, Sephadex gel filtration, and diethylaminoethyl-Sephadex chromatography. The molecular weights of the two forms, by gel filtration, were 50,000 and 25,000. The 50,000-dalton form comprised approximately 84% of the total a-galactosidase activity in crude extracts from mature leaves and was purified 132-fold. The partially purified 25,000molecular weight form rapidly lost activity unless stabilized with 0.2% albumin and accounted for 16% of the total a-galactosidase activity in the crude extract. The smaller molecular weight form was not found in older leaves. The two forms were similar in several ways including their pH optima which were 5.2 and 5.5 for the 50,000- and 25,000-dalton form, respectively, and activation energies, which were 15.4 and 18.9 kilocalories per mole for the larger and smaller forms. Both enzymes were inhibited by galactose as well as by excess concentrations of p-nitrophenyl-a-D-galactoside substrate. Km values with this substrate and with raffinose and melibiose were different for each substrate, but similar for both forms of the enzyme. With stachyose, Km values were 10 and 30 millimolar for the 50,000- and 25,000molecular weight forms, respectively." @default.
- W2515447035 created "2016-09-16" @default.
- W2515447035 creator A5003346899 @default.
- W2515447035 creator A5036066434 @default.
- W2515447035 date "1980-01-01" @default.
- W2515447035 modified "2023-09-27" @default.
- W2515447035 title "Characterization of a-Galactosidase from Cucumber Leaves1" @default.
- W2515447035 cites W1507523846 @default.
- W2515447035 cites W1754420884 @default.
- W2515447035 cites W1775749144 @default.
- W2515447035 cites W1974792635 @default.
- W2515447035 cites W2008308372 @default.
- W2515447035 cites W2015438143 @default.
- W2515447035 cites W2015460175 @default.
- W2515447035 cites W2044605100 @default.
- W2515447035 cites W2074448565 @default.
- W2515447035 hasPublicationYear "1980" @default.
- W2515447035 type Work @default.
- W2515447035 sameAs 2515447035 @default.
- W2515447035 citedByCount "0" @default.
- W2515447035 crossrefType "journal-article" @default.
- W2515447035 hasAuthorship W2515447035A5003346899 @default.
- W2515447035 hasAuthorship W2515447035A5036066434 @default.
- W2515447035 hasConcept C163994747 @default.
- W2515447035 hasConcept C181199279 @default.
- W2515447035 hasConcept C185592680 @default.
- W2515447035 hasConcept C18903297 @default.
- W2515447035 hasConcept C193363816 @default.
- W2515447035 hasConcept C2776392439 @default.
- W2515447035 hasConcept C2777289219 @default.
- W2515447035 hasConcept C2778139943 @default.
- W2515447035 hasConcept C2778173381 @default.
- W2515447035 hasConcept C2778518025 @default.
- W2515447035 hasConcept C2778597767 @default.
- W2515447035 hasConcept C2779998883 @default.
- W2515447035 hasConcept C2780925461 @default.
- W2515447035 hasConcept C43617362 @default.
- W2515447035 hasConcept C55493867 @default.
- W2515447035 hasConcept C86803240 @default.
- W2515447035 hasConcept C97428945 @default.
- W2515447035 hasConceptScore W2515447035C163994747 @default.
- W2515447035 hasConceptScore W2515447035C181199279 @default.
- W2515447035 hasConceptScore W2515447035C185592680 @default.
- W2515447035 hasConceptScore W2515447035C18903297 @default.
- W2515447035 hasConceptScore W2515447035C193363816 @default.
- W2515447035 hasConceptScore W2515447035C2776392439 @default.
- W2515447035 hasConceptScore W2515447035C2777289219 @default.
- W2515447035 hasConceptScore W2515447035C2778139943 @default.
- W2515447035 hasConceptScore W2515447035C2778173381 @default.
- W2515447035 hasConceptScore W2515447035C2778518025 @default.
- W2515447035 hasConceptScore W2515447035C2778597767 @default.
- W2515447035 hasConceptScore W2515447035C2779998883 @default.
- W2515447035 hasConceptScore W2515447035C2780925461 @default.
- W2515447035 hasConceptScore W2515447035C43617362 @default.
- W2515447035 hasConceptScore W2515447035C55493867 @default.
- W2515447035 hasConceptScore W2515447035C86803240 @default.
- W2515447035 hasConceptScore W2515447035C97428945 @default.
- W2515447035 hasLocation W25154470351 @default.
- W2515447035 hasOpenAccess W2515447035 @default.
- W2515447035 hasPrimaryLocation W25154470351 @default.
- W2515447035 hasRelatedWork W1754420884 @default.
- W2515447035 hasRelatedWork W1974652716 @default.
- W2515447035 hasRelatedWork W1997448297 @default.
- W2515447035 hasRelatedWork W2019445527 @default.
- W2515447035 hasRelatedWork W2034432369 @default.
- W2515447035 hasRelatedWork W2039024016 @default.
- W2515447035 hasRelatedWork W2041969841 @default.
- W2515447035 hasRelatedWork W2062594954 @default.
- W2515447035 hasRelatedWork W2076629925 @default.
- W2515447035 hasRelatedWork W2079310652 @default.
- W2515447035 hasRelatedWork W2080299345 @default.
- W2515447035 hasRelatedWork W2080609018 @default.
- W2515447035 hasRelatedWork W2338928806 @default.
- W2515447035 hasRelatedWork W2347932744 @default.
- W2515447035 hasRelatedWork W2349041373 @default.
- W2515447035 hasRelatedWork W2385273008 @default.
- W2515447035 hasRelatedWork W2465455675 @default.
- W2515447035 hasRelatedWork W2514384927 @default.
- W2515447035 hasRelatedWork W2977611380 @default.
- W2515447035 hasRelatedWork W2556919894 @default.
- W2515447035 isParatext "false" @default.
- W2515447035 isRetracted "false" @default.
- W2515447035 magId "2515447035" @default.
- W2515447035 workType "article" @default.