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• W2515530213 abstract "Protein sorting is the fundamental cellular process that creates and maintains cell organelles and subcellular structures. The synaptic vesicle (SV) is a unique cell organelle that contains a plethora of specific SV proteins and its protein composition is crucial for its function. Thus understanding the mechanisms that sort proteins to SVs and other cell organelles is central to neuroscience and cell biology.While in the past protein sorting was studied in the fluorescence and confocal microscope, we here present a protocol that reveals SV protein trafficking and sorting in the electron microscope (EM). The protocol exploits tagging SV proteins with a new genetically encoded label for EM: enhanced horseradish peroxidase (eHRP). eHRP gained its high sensitivity through direct evolution of its catalytic activity and is detectable in the EM and LM after expression in neurons and other mammalian cells. The protocol describes the use of eHRP, labeling of SVs in cultured hippocampal neurons, and analysis via serial section reconstruction." @default.
• W2515530213 created "2016-09-16" @default.
• W2515530213 creator A5087063544 @default.
• W2515530213 date "2016-01-01" @default.
• W2515530213 modified "2023-09-27" @default.
• W2515530213 title "Monitoring Synaptic Vesicle Protein Sorting with Enhanced Horseradish Peroxidase in the Electron Microscope" @default.
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• W2515530213 doi "https://doi.org/10.1007/978-1-4939-6352-2_21" @default.
• W2515530213 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27515091" @default.
• W2515530213 hasPublicationYear "2016" @default.
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