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• W2515565380 endingPage "2922" @default.
• W2515565380 startingPage "2911" @default.
• W2515565380 abstract "The outermost layer of the mammalian skin, the stratum corneum (SC), is a very thin structure and realizes simultaneously the main barrier properties. The penetration barrier for xenobiotica is mostly represented by a complex lipid matrix. There is great interest in the subject of getting information about the arrangement of the lipids, which are mainly ceramides (CER), free fatty acids (FFA) and cholesterol (CHOL). SC lipid model membranes containing synthetically derived lipids in a non-physiological ratio were investigated. To compare the study to a former experiment, a methyl-branched ceramide [EOS] species in presence of the ultra-long chain CER[AP], CHOL and behenic acid (23/10/33/33, wt%) was applied. The membrane structure was studied using the very versatile technique of neutron diffraction. We were able to identify a long-periodicity phase (LPP) with a size of 114 Å or 118 Å with CER[EOS]-br in a ratio of > 60 wt% of the ceramides. Furthermore, we figured out two additional coexisting short-periodicity phases (SPP) with repeat distances of 48 Å and 45 Å, respectively. Partial deuterations of CER[EOS]-br and CER[AP] enabled the localization of the molecules within the multiphase system. CER[EOS]-d3 was present in the LPP, but absent in both SPP. CER[AP]-d3 was determined in both short phases but not localized within the LPP. Besides, we revealed influences of humidity and time with respect to the long-periodicity phase." @default.
• W2515565380 created "2016-09-16" @default.
• W2515565380 creator A5018641992 @default.
• W2515565380 creator A5024349208 @default.
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• W2515565380 creator A5045937197 @default.
• W2515565380 creator A5055675007 @default.
• W2515565380 creator A5084849403 @default.
• W2515565380 date "2016-11-01" @default.
• W2515565380 modified "2023-10-14" @default.
• W2515565380 title "Localization of methyl-branched ceramide [EOS] species within the long-periodicity phase in stratum corneum lipid model membranes: A neutron diffraction study" @default.
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• W2515565380 doi "https://doi.org/10.1016/j.bbamem.2016.09.002" @default.
• W2515565380 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27614192" @default.
• W2515565380 hasPublicationYear "2016" @default.
• W2515565380 type Work @default.
• W2515565380 sameAs 2515565380 @default.

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