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• W2515623196 abstract "We present tRNA-based vectors for producing multiple clustered regularly interspaced short palindromic repeats (CRISPR) single guide RNAs (sgRNAs) from a single RNA polymerase II or III transcript in Drosophila. The system, which is based on liberation of sgRNAs by processing flanking tRNAs, permits highly efficient multiplexing of Cas9-based mutagenesis. We also demonstrate that the tRNA-sgRNA system markedly increases the efficacy of conditional gene disruption by Cas9 and can promote editing by the recently discovered RNA-guided endonuclease Cpf1." @default.
• W2515623196 created "2016-09-16" @default.
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• W2515623196 date "2016-09-05" @default.
• W2515623196 modified "2023-10-18" @default.
• W2515623196 title "Augmenting CRISPR applications in Drosophila with tRNA-flanked sgRNAs" @default.
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• W2515623196 doi "https://doi.org/10.1038/nmeth.3972" @default.
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