FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2515678880> ?p ?o ?g. }

• W2515678880 endingPage "10135" @default.
• W2515678880 startingPage "10126" @default.
• W2515678880 abstract "Cholesterol-containing molecules or nanoparticles play a significant role in achieving favorable plasma membrane imaging and efficient cellular uptake of drugs by the excellent membrane anchoring capability of the cholesterol moiety. By linking cholesterol to a water-soluble component (such as poly(ethylene glycol), PEG), the resulting cholesterol-PEG conjugate can form micelles in aqueous solution through self-assembly, and such a micellar structure represents an important drug delivery vehicle in which hydrophobic drugs can be encapsulated. However, the understanding of the subcellular fate and cytotoxicity of cholesterol-PEG conjugates themselves remains elusive. Herein, by using cholesterol-PEG2000-fluorescein isothiocyanate (Chol-PEG-FITC) as a model system, we found that the Chol-PEG-FITC molecules could attach to the plasma membranes of mammalian cells within 10 min and such a firm membrane attachment could last at least 1 h, displaying excellent plasma membrane staining performance that surpassed that of commonly used commercial membrane dyes such as DiD and CellMask. Besides, we systematically studied the endocytosis pathway and intracellular distribution of Chol-PEG-FITC and found that the cell surface adsorption and endocytosis processes of Chol-PEG-FITC molecules were lipid-raft-dependent. After internalization, the Chol-PEG-FITC molecules gradually reached many organelles with membrane structures. At 5 h, they were mainly distributed in lysosomes and the Golgi apparatus, with some in the endoplasmic reticulum (ER) and very few in the mitochondrion. At 12 h, the Chol-PEG-FITC molecules mostly aggregated in the Golgi apparatus and ER close to the nucleus. Finally, we demonstrated that Chol-PEG-FITC was toxic to mammalian cells only at concentrations above 50 μM. In summary, Chol-PEG-FITC can be a promising plasma membrane imaging reagent to avoid the fast cellular internalization and quick membrane detachment problems faced by commercial membrane dyes. We believe that the investigation of the dynamic subcellular fate of Chol-PEG-FITC can provide important knowledge to facilitate the use of cholesterol–PEG conjugates in fields such as cell surface engineering and drug delivery." @default.
• W2515678880 created "2016-09-16" @default.
• W2515678880 creator A5001037714 @default.
• W2515678880 creator A5003654656 @default.
• W2515678880 creator A5060220945 @default.
• W2515678880 creator A5061691216 @default.
• W2515678880 creator A5065195877 @default.
• W2515678880 date "2016-09-21" @default.
• W2515678880 modified "2023-09-27" @default.
• W2515678880 title "Subcellular Fate of a Fluorescent Cholesterol-Poly(ethylene glycol) Conjugate: An Excellent Plasma Membrane Imaging Reagent" @default.
• W2515678880 cites W1966688342 @default.
• W2515678880 cites W1971207860 @default.
• W2515678880 cites W1972703248 @default.
• W2515678880 cites W1973048821 @default.
• W2515678880 cites W1975957449 @default.
• W2515678880 cites W1981241659 @default.
• W2515678880 cites W1983627025 @default.
• W2515678880 cites W1983777082 @default.
• W2515678880 cites W1984228173 @default.
• W2515678880 cites W1989346639 @default.
• W2515678880 cites W2002936944 @default.
• W2515678880 cites W2004848578 @default.
• W2515678880 cites W2005041817 @default.
• W2515678880 cites W2006156836 @default.
• W2515678880 cites W2006299012 @default.
• W2515678880 cites W2020794186 @default.
• W2515678880 cites W2021414568 @default.
• W2515678880 cites W2022983883 @default.
• W2515678880 cites W2024807954 @default.
• W2515678880 cites W2028432159 @default.
• W2515678880 cites W2028893730 @default.
• W2515678880 cites W2029435311 @default.
• W2515678880 cites W2034947704 @default.
• W2515678880 cites W2036512633 @default.
• W2515678880 cites W2037462412 @default.
• W2515678880 cites W2045635106 @default.
• W2515678880 cites W2054448410 @default.
• W2515678880 cites W2054847598 @default.
• W2515678880 cites W2061424828 @default.
• W2515678880 cites W2065991910 @default.
• W2515678880 cites W2069316295 @default.
• W2515678880 cites W2070554638 @default.
• W2515678880 cites W2072164836 @default.
• W2515678880 cites W2075440313 @default.
• W2515678880 cites W2075915309 @default.
• W2515678880 cites W2077493631 @default.
• W2515678880 cites W2082786939 @default.
• W2515678880 cites W2083100535 @default.
• W2515678880 cites W2088434081 @default.
• W2515678880 cites W2090380610 @default.
• W2515678880 cites W2094811619 @default.
• W2515678880 cites W2101782563 @default.
• W2515678880 cites W2105584431 @default.
• W2515678880 cites W2112101570 @default.
• W2515678880 cites W2113278070 @default.
• W2515678880 cites W2118726922 @default.
• W2515678880 cites W2122633687 @default.
• W2515678880 cites W2140187466 @default.
• W2515678880 cites W2155751481 @default.
• W2515678880 cites W2166041464 @default.
• W2515678880 cites W2169515956 @default.
• W2515678880 cites W2215988569 @default.
• W2515678880 cites W2239302882 @default.
• W2515678880 cites W2261320905 @default.
• W2515678880 cites W2272255765 @default.
• W2515678880 cites W2302981414 @default.
• W2515678880 cites W2317537890 @default.
• W2515678880 cites W2321872032 @default.
• W2515678880 cites W2324830376 @default.
• W2515678880 cites W2328059265 @default.
• W2515678880 cites W2332584645 @default.
• W2515678880 cites W2335364222 @default.
• W2515678880 cites W2346783398 @default.
• W2515678880 cites W2394684459 @default.
• W2515678880 cites W2410603024 @default.
• W2515678880 doi "https://doi.org/10.1021/acs.langmuir.6b02288" @default.
• W2515678880 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27597442" @default.
• W2515678880 hasPublicationYear "2016" @default.
• W2515678880 type Work @default.
• W2515678880 sameAs 2515678880 @default.
• W2515678880 citedByCount "50" @default.
• W2515678880 countsByYear W25156788802017 @default.
• W2515678880 countsByYear W25156788802018 @default.
• W2515678880 countsByYear W25156788802019 @default.
• W2515678880 countsByYear W25156788802020 @default.
• W2515678880 countsByYear W25156788802021 @default.
• W2515678880 countsByYear W25156788802022 @default.
• W2515678880 countsByYear W25156788802023 @default.
• W2515678880 crossrefType "journal-article" @default.
• W2515678880 hasAuthorship W2515678880A5001037714 @default.
• W2515678880 hasAuthorship W2515678880A5003654656 @default.
• W2515678880 hasAuthorship W2515678880A5060220945 @default.
• W2515678880 hasAuthorship W2515678880A5061691216 @default.
• W2515678880 hasAuthorship W2515678880A5065195877 @default.
• W2515678880 hasConcept C10138342 @default.
• W2515678880 hasConcept C102747710 @default.
• W2515678880 hasConcept C11268172 @default.
• W2515678880 hasConcept C119062480 @default.

• next