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- W2516201620 abstract "An inhibitor of cell proliferation was purified from rat liver by alcohol precipitation, ultrafiltration, and DEAE- cellulose chromatography. The hepatic proliferation inhibitor was shown to be pure by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate, analytical isoelectric focusing, and high-performance liquid chromatography. The hepatic pro- liferation inhibitor was found to have a molecular weight of 26,000 and an isoelectric point of 4.65. This protein inhibited the. prolif- eration of nonmalignant rat liver cells in culture, and removal of the protein reversed the inhibition produced by low doses. It ex- erted no effect on the proliferation of malignant rat liver cells. Growth stimulatory factors, nutrients, and ions have been shown to be important in the control of cell proliferation (1-3). A number of factors that stimulate cell proliferation have been purified from various tissues and are well characterized. In- deed, many of these purified growth factors are commercially available and are being used extensively to investigate various aspects of cell biology. In contrast, little is known about the role of growth inhibitory factors, although they clearly are involved in the control of cell proliferation which itself is critical in the carcinogenic process as discussed in depth by Potter (4, 5). Considerable literature is available on the existence of inhibitors of cell proliferation in different cells and tissues (6-15), but such factors from normal tissues have not been purified." @default.
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- W2516201620 date "2016-01-01" @default.
- W2516201620 modified "2023-09-27" @default.
- W2516201620 title "(hepatic proliferation inhibitor/high-performance liquid chromatogrs" @default.
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