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• W2516317699 abstract "Patients with a heterozygous mutation in the gene encoding the transcription factor, PAX6, have a degenerative corneal opacity associated with failure of normal radial epithelial cell migration across the corneal surface and a reported wound healing defect. This study investigated the guidance mechanisms that drive the directed migration of corneal epithelial cells.In vivo corneal epithelial wounding was performed in adult wild-type and Pax6(+/-) mice, and the healing migration rates were compared. To investigate the control of the cell migration direction, primary corneal epithelial cells from wild-type and Pax6(+/-) mice were plated on grooved quartz substrates, and alignment relative to the grooves was assayed. A reconstructed corneal culture system was developed in which dissociated wild-type and genetically mutant corneal epithelial cells could be cultured on a de-epithelialized corneal stroma or basement membrane and their migration assayed with time-lapse microscopy.The Pax6(+/-) cells efficiently re-epithelialized corneal wounds in vivo but had mild slowing of healing migration compared to the wild-type. Cells aligned parallel to quartz grooves in vitro, but the Pax6(+/-) cells were less robustly oriented than the wild-type. In the reconstructed corneal culture system, corneal epithelial cells continued to migrate radially, showing that the cells are guided by contact-mediated cues from the basement membrane. Recombining wild-type and Pax6 mutant corneal epithelial cells with wild-type and Pax6 mutant corneal stroma showed that normal Pax6 dosage was required autonomously in the epithelial cells for directed migration. Integrin-mediated attachment to the substrate, and intracellular PI3Kγ activity, were required for migration. Pharmacological inhibition of cAMP signaling randomized migration tracks in reconstructed corneas.Striking patterns of centripetal migration of corneal epithelial cells observed in vivo are driven by contact-mediated cues operating through an intracellular cAMP pathway, and failure to read these cues underlies the migration defects that accompany corneal degeneration in patients with mutations in PAX6." @default.
• W2516317699 created "2016-09-16" @default.
• W2516317699 creator A5028498863 @default.
• W2516317699 creator A5032984696 @default.
• W2516317699 creator A5082081788 @default.
• W2516317699 date "2016-01-01" @default.
• W2516317699 modified "2023-09-23" @default.
• W2516317699 title "Contact-mediated control of radial migration of corneal epithelial cells." @default.
• W2516317699 cites W113450198 @default.
• W2516317699 cites W135166961 @default.
• W2516317699 cites W1496069677 @default.
• W2516317699 cites W149922413 @default.
• W2516317699 cites W1515583925 @default.
• W2516317699 cites W1963892111 @default.
• W2516317699 cites W1965438871 @default.
• W2516317699 cites W1967470122 @default.
• W2516317699 cites W196823532 @default.
• W2516317699 cites W1970516512 @default.
• W2516317699 cites W1971325550 @default.
• W2516317699 cites W1972420511 @default.
• W2516317699 cites W1976837011 @default.
• W2516317699 cites W1977075406 @default.
• W2516317699 cites W1978609960 @default.
• W2516317699 cites W1978818002 @default.
• W2516317699 cites W1987890424 @default.
• W2516317699 cites W1990804592 @default.
• W2516317699 cites W1994538451 @default.
• W2516317699 cites W1996651169 @default.
• W2516317699 cites W1997501499 @default.
• W2516317699 cites W1998578954 @default.
• W2516317699 cites W1999295970 @default.
• W2516317699 cites W2001602989 @default.
• W2516317699 cites W2004136067 @default.
• W2516317699 cites W2009514690 @default.
• W2516317699 cites W2011054586 @default.
• W2516317699 cites W2014421736 @default.
• W2516317699 cites W2014794009 @default.
• W2516317699 cites W2028242823 @default.
• W2516317699 cites W2030153375 @default.
• W2516317699 cites W2035234962 @default.
• W2516317699 cites W2036139614 @default.
• W2516317699 cites W2036914065 @default.
• W2516317699 cites W2039016753 @default.
• W2516317699 cites W2045856913 @default.
• W2516317699 cites W2051033440 @default.
• W2516317699 cites W2053305914 @default.
• W2516317699 cites W2054721944 @default.
• W2516317699 cites W2058453423 @default.
• W2516317699 cites W2061440101 @default.
• W2516317699 cites W2067920606 @default.
• W2516317699 cites W2075722086 @default.
• W2516317699 cites W2080992511 @default.
• W2516317699 cites W2085988624 @default.
• W2516317699 cites W2086316557 @default.
• W2516317699 cites W2087429877 @default.
• W2516317699 cites W2087438030 @default.
• W2516317699 cites W2087661168 @default.
• W2516317699 cites W2091421632 @default.
• W2516317699 cites W2094328201 @default.
• W2516317699 cites W2095034102 @default.
• W2516317699 cites W2104634592 @default.
• W2516317699 cites W2104908771 @default.
• W2516317699 cites W2109582895 @default.
• W2516317699 cites W2113490614 @default.
• W2516317699 cites W2114923998 @default.
• W2516317699 cites W2118661742 @default.
• W2516317699 cites W2125044120 @default.
• W2516317699 cites W2128078747 @default.
• W2516317699 cites W2133032561 @default.
• W2516317699 cites W2134940624 @default.
• W2516317699 cites W2137898020 @default.
• W2516317699 cites W2139103260 @default.
• W2516317699 cites W2148304597 @default.
• W2516317699 cites W2148308138 @default.
• W2516317699 cites W2153571086 @default.
• W2516317699 cites W2154040587 @default.
• W2516317699 cites W2158671243 @default.
• W2516317699 cites W2163579392 @default.
• W2516317699 cites W2163674890 @default.
• W2516317699 cites W2167652900 @default.
• W2516317699 cites W2168534525 @default.
• W2516317699 cites W2168960379 @default.
• W2516317699 cites W2171696866 @default.
• W2516317699 cites W2328549167 @default.
• W2516317699 cites W2404646478 @default.
• W2516317699 cites W2536400325 @default.
• W2516317699 cites W2953375613 @default.
• W2516317699 cites W44512059 @default.
• W2516317699 cites W49279459 @default.
• W2516317699 cites W7971299 @default.
• W2516317699 cites W905152366 @default.
• W2516317699 cites W2069326128 @default.
• W2516317699 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4976620" @default.
• W2516317699 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27563231" @default.
• W2516317699 hasPublicationYear "2016" @default.
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