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- W2516658001 abstract "Artificial production of haploids is one of the important sought-after goals of plant breeding and crop improvement programs. Conventionally, haploid plants are generated by in vitro (tissue) culture of haploid plant gametophytes, pollen (male), and embryo sac (female). Here, we describe a facile, nontissue culture-based in vivo method of haploid production through seeds in the model plant, Arabidopsis thaliana. This method involves simple crossing of any desired genotype of interest to a haploid-inducing strain (GFP-tailswap) to directly obtain haploid F1 seeds. The described protocol can be practiced by anyone with basic experience in growing A. thaliana plants and will be of interest to Arabidopsis research community." @default.
- W2516658001 created "2016-09-16" @default.
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- W2516658001 date "2016-01-01" @default.
- W2516658001 modified "2023-09-27" @default.
- W2516658001 title "Genome Elimination by Tailswap CenH3: In Vivo Haploid Production in Arabidopsis thaliana" @default.
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- W2516658001 doi "https://doi.org/10.1007/978-1-4939-4931-1_6" @default.
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