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- W2517016145 abstract "A new method for flow cytometric analysis of calcium mobilization in human peripheral blood basophils without prior purification was developed. The method is based on dual color analysis of centrifugation-enriched mononuclear cell populations using fluo-3 and phycoerythrin (PE)-conjugated CD2, CD14, CD16, CD19 monoclonal antibodies (mAb) to stain contaminated cells. This technique allows the detection of fluo-3 fluorescence as a measure of an increase in the cytoplasmic free calcium concentration ( [Ca2+] 1) while simultaneously discriminating PE-mAb-unlabelled basophils.To clarify whether the human peripheral blood basophil is activated through the low affinity IgG receptor, Fc γ RII, as well as the high affinity IgE receptor, Fc ε R I, calcium mobilization after Fc γ RII stimulation was analyzed by this method. After cross-linking of Fc γ RII, transient [Ca2+] 1 elevation was observed but there was no apparent difference with interleukin-3 (IL-3)-treated cells, and no significant histamine release was observed with or without short pre-incubation of IL-3. These findings suggest that the cross-linking of Fc γ RII, not only Fc ε R I, can activate human basophils which may result in mediator release other than histamine." @default.
- W2517016145 created "2016-09-16" @default.
- W2517016145 creator A5045148503 @default.
- W2517016145 date "1994-01-01" @default.
- W2517016145 modified "2023-10-16" @default.
- W2517016145 title "The mechanism of human basophil activation through the low affinity IgG receptor (Fc γ RII)" @default.
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- W2517016145 doi "https://doi.org/10.4044/joma1947.106.7-8_847" @default.
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