FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2517975101> ?p ?o ?g. }

• W2517975101 endingPage "1558" @default.
• W2517975101 startingPage "1547" @default.
• W2517975101 abstract "The biopharmaceutical industry has invested considerably in the implementation of single-use disposable bioreactors in place of or in addition to their stainless steel-counterparts. This new wave of construction materials for disposable bioprocess containers encompass a plethora of uncharacterized secondary compounds that, when in contact with the culture media, can leach, contaminating the bioprocess. One such cytotoxic leachable already receiving attention is bis(2,4-di-tert-butylphenyl)-phosphate (bDtBPP), a breakdown product of the secondary antioxidant Irgafos 168 in polyethylene-film based bags. This compound has been demonstrated to inhibit cell growth at concentrations ranging from 0.12 to 0.73 mg/L across an array of cell lines. Here we demonstrate that a further two CHO cell lines exhibit sensitivity to bDtBPP exposure at concentrations lower than that previously reported (0.035-0.1 mg/L). Furthermore, these inhibitory concentrations reflect bDtBPP levels found to leach early into the bioprocess, exposing reactor inoculums to serious risk. Quantitative label-free LC-MS/MS revealed that irrespective of cell line or concentration of bDtBPP, 8 proteins were found to be commonly differentially expressed in response to exposure to the compound highlighting biological processes related to cellular stress. Although the glycoprofile of the recombinant antibody remains primarily unchanged, we demonstrate that this compound when spiked at meaningful concentrations 72 h into culture considerably reduces the maximum cell density achieved. Studies like this reinforce the requirement for the complete characterization of all potential leachable compounds from disposable materials to assess their risk not only to the patient but also to the production pipeline itself. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1547-1558, 2016." @default.
• W2517975101 created "2016-09-16" @default.
• W2517975101 creator A5010144309 @default.
• W2517975101 creator A5013224445 @default.
• W2517975101 creator A5018053019 @default.
• W2517975101 creator A5018463575 @default.
• W2517975101 creator A5039366298 @default.
• W2517975101 creator A5046090387 @default.
• W2517975101 creator A5061707602 @default.
• W2517975101 creator A5070489124 @default.
• W2517975101 creator A5077091444 @default.
• W2517975101 creator A5079805292 @default.
• W2517975101 creator A5088540624 @default.
• W2517975101 date "2016-09-04" @default.
• W2517975101 modified "2023-09-27" @default.
• W2517975101 title "Process-relevant concentrations of the leachable bDtBPP impact negatively on CHO cell production characteristics" @default.
• W2517975101 cites W1485008941 @default.
• W2517975101 cites W1841003668 @default.
• W2517975101 cites W1894135252 @default.
• W2517975101 cites W1950250567 @default.
• W2517975101 cites W1985274289 @default.
• W2517975101 cites W1991233540 @default.
• W2517975101 cites W2013004443 @default.
• W2517975101 cites W2015481043 @default.
• W2517975101 cites W2018929792 @default.
• W2517975101 cites W2022296588 @default.
• W2517975101 cites W2043134556 @default.
• W2517975101 cites W2044925931 @default.
• W2517975101 cites W2050640183 @default.
• W2517975101 cites W2052204213 @default.
• W2517975101 cites W2056034615 @default.
• W2517975101 cites W2058386777 @default.
• W2517975101 cites W2060964592 @default.
• W2517975101 cites W2085270297 @default.
• W2517975101 cites W2101023381 @default.
• W2517975101 cites W2104772632 @default.
• W2517975101 cites W2106691841 @default.
• W2517975101 cites W2114822038 @default.
• W2517975101 cites W2117839670 @default.
• W2517975101 cites W2120002250 @default.
• W2517975101 cites W2130635082 @default.
• W2517975101 cites W2133740757 @default.
• W2517975101 cites W2155377925 @default.
• W2517975101 cites W2156748257 @default.
• W2517975101 cites W2298210917 @default.
• W2517975101 cites W2410993730 @default.
• W2517975101 doi "https://doi.org/10.1002/btpr.2345" @default.
• W2517975101 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27557043" @default.
• W2517975101 hasPublicationYear "2016" @default.
• W2517975101 type Work @default.
• W2517975101 sameAs 2517975101 @default.
• W2517975101 citedByCount "28" @default.
• W2517975101 countsByYear W25179751012017 @default.
• W2517975101 countsByYear W25179751012018 @default.
• W2517975101 countsByYear W25179751012019 @default.
• W2517975101 countsByYear W25179751012020 @default.
• W2517975101 countsByYear W25179751012021 @default.
• W2517975101 countsByYear W25179751012022 @default.
• W2517975101 countsByYear W25179751012023 @default.
• W2517975101 crossrefType "journal-article" @default.
• W2517975101 hasAuthorship W2517975101A5010144309 @default.
• W2517975101 hasAuthorship W2517975101A5013224445 @default.
• W2517975101 hasAuthorship W2517975101A5018053019 @default.
• W2517975101 hasAuthorship W2517975101A5018463575 @default.
• W2517975101 hasAuthorship W2517975101A5039366298 @default.
• W2517975101 hasAuthorship W2517975101A5046090387 @default.
• W2517975101 hasAuthorship W2517975101A5061707602 @default.
• W2517975101 hasAuthorship W2517975101A5070489124 @default.
• W2517975101 hasAuthorship W2517975101A5077091444 @default.
• W2517975101 hasAuthorship W2517975101A5079805292 @default.
• W2517975101 hasAuthorship W2517975101A5088540624 @default.
• W2517975101 hasConcept C1491633281 @default.
• W2517975101 hasConcept C150903083 @default.
• W2517975101 hasConcept C151730666 @default.
• W2517975101 hasConcept C168170006 @default.
• W2517975101 hasConcept C170493617 @default.
• W2517975101 hasConcept C175656101 @default.
• W2517975101 hasConcept C178790620 @default.
• W2517975101 hasConcept C185592680 @default.
• W2517975101 hasConcept C2778004101 @default.
• W2517975101 hasConcept C2781047461 @default.
• W2517975101 hasConcept C31903555 @default.
• W2517975101 hasConcept C43617362 @default.
• W2517975101 hasConcept C4916135 @default.
• W2517975101 hasConcept C54355233 @default.
• W2517975101 hasConcept C55493867 @default.
• W2517975101 hasConcept C61664519 @default.
• W2517975101 hasConcept C62112901 @default.
• W2517975101 hasConcept C81885089 @default.
• W2517975101 hasConcept C86803240 @default.
• W2517975101 hasConceptScore W2517975101C1491633281 @default.
• W2517975101 hasConceptScore W2517975101C150903083 @default.
• W2517975101 hasConceptScore W2517975101C151730666 @default.
• W2517975101 hasConceptScore W2517975101C168170006 @default.
• W2517975101 hasConceptScore W2517975101C170493617 @default.
• W2517975101 hasConceptScore W2517975101C175656101 @default.
• W2517975101 hasConceptScore W2517975101C178790620 @default.
• W2517975101 hasConceptScore W2517975101C185592680 @default.

• next