FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2517993883> ?p ?o ?g. }

• W2517993883 endingPage "21540" @default.
• W2517993883 startingPage "21531" @default.
• W2517993883 abstract "GlgE is a maltosyltransferase involved in α-glucan biosynthesis in bacteria that has been genetically validated as a target for tuberculosis therapies. Crystals of the Mycobacterium tuberculosis enzyme diffract at low resolution so most structural studies have been with the very similar Streptomyces coelicolor GlgE isoform 1. Although the donor binding site for α-maltose 1-phosphate had been previously structurally defined, the acceptor site had not. Using mutagenesis, kinetics, and protein crystallography of the S. coelicolor enzyme, we have now identified the +1 to +6 subsites of the acceptor/product, which overlap with the known cyclodextrin binding site. The sugar residues in the acceptor subsites +1 to +5 are oriented such that they disfavor the binding of malto-oligosaccharides that bear branches at their 6-positions, consistent with the known acceptor chain specificity of GlgE. A secondary binding site remote from the catalytic center was identified that is distinct from one reported for the M. tuberculosis enzyme. This new site is capable of binding a branched α-glucan and is most likely involved in guiding acceptors toward the donor site because its disruption kinetically compromises the ability of GlgE to extend polymeric substrates. However, disruption of this site, which is conserved in the Streptomyces venezuelae GlgE enzyme, did not affect the growth of S. venezuelae or the structure of the polymeric product. The acceptor subsites +1 to +4 in the S. coelicolor enzyme are well conserved in the M. tuberculosis enzyme so their identification could help inform the design of inhibitors with therapeutic potential." @default.
• W2517993883 created "2016-09-16" @default.
• W2517993883 creator A5004862128 @default.
• W2517993883 creator A5019918068 @default.
• W2517993883 creator A5021319045 @default.
• W2517993883 creator A5048506881 @default.
• W2517993883 creator A5054991307 @default.
• W2517993883 creator A5059885629 @default.
• W2517993883 creator A5072890871 @default.
• W2517993883 creator A5074458795 @default.
• W2517993883 creator A5076156885 @default.
• W2517993883 date "2016-10-01" @default.
• W2517993883 modified "2023-10-13" @default.
• W2517993883 title "Ligand-bound Structures and Site-directed Mutagenesis Identify the Acceptor and Secondary Binding Sites of Streptomyces coelicolor Maltosyltransferase GlgE" @default.
• W2517993883 cites W1892158199 @default.
• W2517993883 cites W1952600751 @default.
• W2517993883 cites W1966729619 @default.
• W2517993883 cites W1971094557 @default.
• W2517993883 cites W1976491393 @default.
• W2517993883 cites W1989689041 @default.
• W2517993883 cites W1994051007 @default.
• W2517993883 cites W1996343958 @default.
• W2517993883 cites W2006922696 @default.
• W2517993883 cites W2008402427 @default.
• W2517993883 cites W2012089496 @default.
• W2517993883 cites W2021490051 @default.
• W2517993883 cites W2023950913 @default.
• W2517993883 cites W2031433499 @default.
• W2517993883 cites W2036216887 @default.
• W2517993883 cites W2038469571 @default.
• W2517993883 cites W2038840577 @default.
• W2517993883 cites W2052163957 @default.
• W2517993883 cites W2055279550 @default.
• W2517993883 cites W2059750011 @default.
• W2517993883 cites W2067921100 @default.
• W2517993883 cites W2076163976 @default.
• W2517993883 cites W2090028899 @default.
• W2517993883 cites W2094784562 @default.
• W2517993883 cites W2109184569 @default.
• W2517993883 cites W2110958200 @default.
• W2517993883 cites W2122339645 @default.
• W2517993883 cites W2122559203 @default.
• W2517993883 cites W2133290304 @default.
• W2517993883 cites W2133702031 @default.
• W2517993883 cites W2144081223 @default.
• W2517993883 cites W2148033508 @default.
• W2517993883 cites W2152190430 @default.
• W2517993883 cites W2163341755 @default.
• W2517993883 cites W2186662477 @default.
• W2517993883 cites W2254996396 @default.
• W2517993883 cites W2324519019 @default.
• W2517993883 cites W2332241546 @default.
• W2517993883 cites W2337372479 @default.
• W2517993883 cites W2344619545 @default.
• W2517993883 cites W2398151672 @default.
• W2517993883 cites W2511376133 @default.
• W2517993883 doi "https://doi.org/10.1074/jbc.m116.748160" @default.
• W2517993883 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5076824" @default.
• W2517993883 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27531751" @default.
• W2517993883 hasPublicationYear "2016" @default.
• W2517993883 type Work @default.
• W2517993883 sameAs 2517993883 @default.
• W2517993883 citedByCount "11" @default.
• W2517993883 countsByYear W25179938832017 @default.
• W2517993883 countsByYear W25179938832019 @default.
• W2517993883 countsByYear W25179938832020 @default.
• W2517993883 countsByYear W25179938832021 @default.
• W2517993883 countsByYear W25179938832022 @default.
• W2517993883 countsByYear W25179938832023 @default.
• W2517993883 crossrefType "journal-article" @default.
• W2517993883 hasAuthorship W2517993883A5004862128 @default.
• W2517993883 hasAuthorship W2517993883A5019918068 @default.
• W2517993883 hasAuthorship W2517993883A5021319045 @default.
• W2517993883 hasAuthorship W2517993883A5048506881 @default.
• W2517993883 hasAuthorship W2517993883A5054991307 @default.
• W2517993883 hasAuthorship W2517993883A5059885629 @default.
• W2517993883 hasAuthorship W2517993883A5072890871 @default.
• W2517993883 hasAuthorship W2517993883A5074458795 @default.
• W2517993883 hasAuthorship W2517993883A5076156885 @default.
• W2517993883 hasBestOaLocation W25179938831 @default.
• W2517993883 hasConcept C103408237 @default.
• W2517993883 hasConcept C104317684 @default.
• W2517993883 hasConcept C107824862 @default.
• W2517993883 hasConcept C143065580 @default.
• W2517993883 hasConcept C16318435 @default.
• W2517993883 hasConcept C181199279 @default.
• W2517993883 hasConcept C185592680 @default.
• W2517993883 hasConcept C2779920670 @default.
• W2517993883 hasConcept C2780681812 @default.
• W2517993883 hasConcept C41183919 @default.
• W2517993883 hasConcept C523546767 @default.
• W2517993883 hasConcept C54355233 @default.
• W2517993883 hasConcept C55493867 @default.
• W2517993883 hasConcept C71240020 @default.
• W2517993883 hasConcept C86803240 @default.
• W2517993883 hasConceptScore W2517993883C103408237 @default.
• W2517993883 hasConceptScore W2517993883C104317684 @default.
• W2517993883 hasConceptScore W2517993883C107824862 @default.

• next