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- W2517998006 abstract "The nuclear transfer technique is a tool that makes possible the production of embryos clones that can be used to provide the birth of identical animals or as model for the study of several physiologic mechanisms, during the embryonic development. In this way, bovine embryos clones were produced by nuclear transfer with the objective of establishing the cloning technique, as well as to optimize it at the conditions of routine work at the Laboratory of Embryology and Biotechnics of Reproduction of UFRGS. During this work, 1.123 structures were reconstructed in different conditions, and 95 blastocysts were produced, in 56 replicate procedures. The first blastocyst was produced in the 5th routine. After the transfer of part of these embryos, 13 pregnancies were established, however, most of them was lost at the beginning of pregnancy. A twin pregnancy reached 260 days, however the fetuses were miscarried. Another gestation was to term, with the birth by Cesarian of an alive clone, however the animal came to death 42 hours after the birth." @default.
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- W2517998006 date "2008-01-01" @default.
- W2517998006 modified "2023-09-23" @default.
- W2517998006 title "Otimização do sistema de produção de clones por transferência nuclear de célula somática (NTSC) Cloning by Somatic Cell Nuclear Transfer - Procedures optimization" @default.
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