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- W2517998519 abstract "We herein developed a method that required a smaller sample volume to rapidly determine plasma stiripentol (STP) concentrations in patients with Dravet syndrome by means of reverse-phase high-performance liquid chromatography (HPLC) using a fluorescence detector (Ex/Em: 210/400 nm). A pretreatment involved simple deproteinization with acetonitrile. A Discovery® HS C18 column, 3 μm, 4.6 mm × 150 mm was used for isolation by HPLC. The mobile phase, consisting of 25 mM phosphate buffer (pH 2.6) and acetonitrile (43:57, v/v), had a flow rate of 1.5 mL/min. The retention time was 4.6 minutes, and the lower limit of quantification was 0.05 μg/mL. Specificity testing revealed no influence on the peak. A plasma sample of 10 μL was sufficient to measure plasma STP concentrations in patients with Dravet syndrome; thus, the burden on these patients was markedly reduced. These results suggest that the determination of plasma STP concentrations was useful." @default.
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- W2517998519 date "2015-01-01" @default.
- W2517998519 modified "2023-10-06" @default.
- W2517998519 title "Determination of Stiripentol in Plasma by High-performance Liquid Chromatography with Fluorescence Detection" @default.
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- W2517998519 doi "https://doi.org/10.5649/jjphcs.41.643" @default.
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