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• W2518137818 startingPage "3073" @default.
• W2518137818 abstract "Mulberry badnavirus 1 (MBV1) has been characterized as the aetiological agent of a disease observed on a mulberry tree in Lebanon (accession L34). A small RNA next-generation sequencing library was prepared and analysed from L34 extract, and these data together with genome walking experiments have been used to obtain the full-length virus sequence. Uniquely among badnaviruses, the MBV1 sequence encodes a single ORF containing all the conserved pararetrovirus motifs. Two genome sizes (6 kb and 7 kb) were found to be encapsidated in infected plants, the shortest of which shares 98.95 % sequence identity with the full L34 genome. In the less-than-full-length deleted genome, the translational frame for the replication domains was conserved, but the particle morphology, observed under electron microscopy, was somehow altered. Southern blot hybridization confirmed the coexistence of the two genomic forms in the original L34 accession, as well as the absence of cointegration in the plant genome. Both long and deleted genomes were cloned and proved to be infectious in mulberry. Differently from other similar nuclear-replicating viruses or viroids, the characterization of the MBV1-derived small RNAs showed a reduced amount of the 24-mer class size." @default.
• W2518137818 created "2016-09-16" @default.
• W2518137818 creator A5007150931 @default.
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• W2518137818 date "2016-11-10" @default.
• W2518137818 modified "2023-10-03" @default.
• W2518137818 title "Unusual genomic features of a badnavirus infecting mulberry" @default.
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• W2518137818 doi "https://doi.org/10.1099/jgv.0.000600" @default.
• W2518137818 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27604547" @default.
• W2518137818 hasPublicationYear "2016" @default.
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