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- W2518214728 abstract "Hypochlorite (ClO–) and glutathione (GSH) have been reported to closely correlate with oxidative stress and related diseases; however, a clear mechanism is still unknown, mainly owing to a lack of accurate analytical methods for live cells. Herein we create a novel surface-enhanced Raman scattering (SERS) nanoprobe, 4-mercaptophenol (4-MP)-functionalized gold flowers (AuF/MP), for imaging and biosensing of ClO– and GSH in RAW 264.7 macrophage cells upon oxidative stress. The SERS spectra of AuF/MP change with the reaction between ClO– and 4-MP on AuFs within 1 min and then recover after reaction with GSH, resulting in the ratiometric detection of ClO– and GSH with high accuracy. The single SERS probe also shows high selectivity for ClO– and GSH detection against other reactive oxygen species and amino acids which may exist in biological systems, as well as remarkable sensitivity ascribed to a larger amount of hot spots on AuFs. The significant analytical performance of the developed nanoprobe, together with good biocompatibility and high cell-permeability, enables the present SERS probe imaging and real-time detection of ClO– and GSH in live cells upon oxidative stress." @default.
- W2518214728 created "2016-09-16" @default.
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- W2518214728 date "2016-09-16" @default.
- W2518214728 modified "2023-10-17" @default.
- W2518214728 title "A Single Nanoprobe for Ratiometric Imaging and Biosensing of Hypochlorite and Glutathione in Live Cells Using Surface-Enhanced Raman Scattering" @default.
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- W2518214728 doi "https://doi.org/10.1021/acs.analchem.6b02081" @default.
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