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- W2518360084 abstract "CRABS CLAW( CRC) is a member of the YABBYA transcription factor gene family that plays an important role in floral organ development of plants.This study aimed to further investigate the regulatory function of CRC transcription factor in the development of floral organs of rape( Brassica napus L.). A 580 bp fragment of CRC gene was cloned by RT-PCR from total RNA of buds of rape cultivar Ningyou No. 10 to construct an inverted repeated expression cassette of CRC gene using intermediate vector p Hurricane. Firstly,CRC gene fragment was positively inserted into the 5’end of a spliceable intron and negatively inserted into the3’ end of the intron. Subsequently,Ca MV35 S promoter sequence and inverted repeated expression cassette of CRC gene were transferred into p UC18 multiple clone site of binary expression vector p CAMBIAI1390. The constructed interference expression vector was named p A6-CRCi,which was further confirmed by restriction enzyme digestion and sequencing." @default.
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- W2518360084 date "2016-01-01" @default.
- W2518360084 modified "2023-09-24" @default.
- W2518360084 title "Cloning of CRABS CLAW Gene from Brassica napus and Construction of Its RNA Interference Expression Vector" @default.
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